Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Applications of Molecular Taxonomy01:20

Applications of Molecular Taxonomy

265
Molecular taxonomy has revolutionized the understanding and classification of bacteria, providing precise insights into their diversity, evolutionary relationships, and ecological roles. By utilizing molecular techniques such as DNA sequencing and fingerprinting, researchers have made significant strides in various fields related to bacterial studies.Resolving Taxonomic AmbiguitiesMolecular taxonomy has been instrumental in distinguishing closely related bacterial species initially thought to...
265
Modern Molecular Taxonomy01:29

Modern Molecular Taxonomy

329
Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...
329

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Enteropathy-associated T-cell lymphoma: molecular clonal evolution and its clinical consequences.

Haematologica·2026
Same author

A new <i>Tetranchyroderma</i> species (Gastrotricha, Macrodasyida) from Tunisia, with its phylogenetic position based on molecular markers.

Zootaxa·2026
Same author

Invisible invaders: A new warning flag for molecularly detected alien species (MODAS) in databases and information systems.

Ecological applications : a publication of the Ecological Society of America·2026
Same author

The global diversity and decline of glacier animals.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Nodular lymphocyte-predominant Hodgkin lymphoma controversy and insights: a global NLPHL one working group summit report.

Leukemia·2026
Same author

Multilayered Human Activities Shape the Microbial Communities of Groundwater-Dependent Ecosystems on an Arid Oceanic Island.

Microbial ecology·2026

Related Experiment Video

Updated: Nov 6, 2025

A Rapid Method to Confine and Safely Handle Bees in the Field
03:45

A Rapid Method to Confine and Safely Handle Bees in the Field

Published on: August 23, 2024

746

Enhancing metabarcoding efficiency and ecological insights through integrated taxonomy and DNA reference barcoding:

Jan-Niklas Macher1,2, Alejandro Martínez3, Sude Çakir1

  • 1Naturalis Biodiversity Center, Marine Biodiversity, Leiden, The Netherlands.

Molecular Ecology Resources
|August 1, 2024
PubMed
Summary

Combining DNA barcoding and morphological taxonomy significantly enhances metabarcoding efficiency for assessing beach meiofauna biodiversity. This approach doubles species identification and improves sequence annotation for better coastal ecosystem understanding.

Keywords:
DNA barcodingMolecular reference databasecommunity ecologyinvertebrates

More Related Videos

Empirical, Metagenomic, and Computational Techniques Illuminate the Mechanisms by which Fungicides Compromise Bee Health
08:36

Empirical, Metagenomic, and Computational Techniques Illuminate the Mechanisms by which Fungicides Compromise Bee Health

Published on: October 9, 2017

9.7K
Simultaneous DNA-RNA Extraction from Coastal Sediments and Quantification of 16S rRNA Genes and Transcripts by Real-time PCR
11:37

Simultaneous DNA-RNA Extraction from Coastal Sediments and Quantification of 16S rRNA Genes and Transcripts by Real-time PCR

Published on: June 11, 2016

17.6K

Related Experiment Videos

Last Updated: Nov 6, 2025

A Rapid Method to Confine and Safely Handle Bees in the Field
03:45

A Rapid Method to Confine and Safely Handle Bees in the Field

Published on: August 23, 2024

746
Empirical, Metagenomic, and Computational Techniques Illuminate the Mechanisms by which Fungicides Compromise Bee Health
08:36

Empirical, Metagenomic, and Computational Techniques Illuminate the Mechanisms by which Fungicides Compromise Bee Health

Published on: October 9, 2017

9.7K
Simultaneous DNA-RNA Extraction from Coastal Sediments and Quantification of 16S rRNA Genes and Transcripts by Real-time PCR
11:37

Simultaneous DNA-RNA Extraction from Coastal Sediments and Quantification of 16S rRNA Genes and Transcripts by Real-time PCR

Published on: June 11, 2016

17.6K

Area of Science:

  • Marine Biology
  • Ecology
  • Molecular Ecology

Background:

  • Metabarcoding is vital for community diversity studies but limited by reference DNA sequences.
  • Beach meiofauna are crucial for coastal ecosystems but face research challenges due to taxonomic and sampling limitations.

Purpose of the Study:

  • To improve metabarcoding efficiency for biodiversity assessment by combining DNA barcoding and morphological taxonomy.
  • To investigate the impact of an enriched reference database on analyzing beach meiofauna communities.

Main Methods:

  • Generated 775 new cytochrome c oxidase I DNA barcodes from meiofauna.
  • Combined new barcodes with NCBI GenBank database to create an enriched reference library.
  • Analyzed alpha and beta diversity in 561 metabarcoding samples using both enriched and standard databases.

Main Results:

  • Achieved a 2.5-fold increase in sequence annotation and doubled species-level Operational Taxonomic Units (OTUs) identification with the enriched database.
  • Observed a bell-shaped OTU richness curve across the intertidal zone, aligning with morphological data.
  • Identified clearer community dissimilarity patterns between supralittoral and intertidal sites.

Conclusions:

  • Expanding molecular reference databases is crucial for accurate biodiversity assessments.
  • Integrating morphological taxonomy with molecular techniques enhances metabarcoding efficiency and ecological understanding.
  • This combined approach improves the study of coastal ecosystems and their biodiversity.