Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Cell Polarization by Rho Proteins01:21

Cell Polarization by Rho Proteins

2.7K
Cell polarity is the asymmetric distribution of cellular and membrane components, making one side of the cell different from the other. This polarity is essential to many processes such as embryogenesis, axon migration, glucose transport across epithelial cells, and directional cell migration. A migrating cell responds to intracellular or extracellular signals via molecular cascades that reorganize the actin cytoskeleton to establish this polarity. In these cells, the Rho family proteins Cdc42,...
2.7K
Tight Junctions01:29

Tight Junctions

5.2K
Tight junctions are molecular seals between cells that prevent the leaking of fluids, ions, and other small solutes across cavities and compartments in multicellular organisms. They are mainly composed of claudin and occludin transmembrane proteins, and other proteins such as tricellulin and JAM (junctional adhesion molecule). All these proteins are 4-pass transmembrane proteins, except JAM, which is a single-pass transmembrane protein belonging to the immunoglobulin superfamily. The...
5.2K
Cytoskeletal Coordination in Cell Migration01:32

Cytoskeletal Coordination in Cell Migration

4.7K
A migrating cell changes its shape during the cyclic events of attachment and detachment from the substratum and repositions the cell organelles correspondingly. These complex events are orchestrated by the dynamic cytoskeletal network comprising actin filaments, intermediate filaments, and microtubules. Cytoskeletal crosstalk — the direct and indirect communication between the different components — is crucial for this coordination. Direct communication involves various linker...
4.7K
Cell-matrix's Response to Mechanical Forces01:13

Cell-matrix's Response to Mechanical Forces

2.6K
In animal cells, the extracellular matrix allows cells within tissues to withstand external stresses and transmits signals from the outside of the cell to the inside. The extracellular matrix is extensive, and its composition varies between different types of tissues. For example, the reticular fibers and ground substance make up the ECM in loose connective tissue, while collagen and bone minerals make up the ECM of bone tissue. 
Anchoring junctions mechanically attach a cell to the...
2.6K
Anchoring Junctions01:03

Anchoring Junctions

3.7K
Anchoring junctions are multiprotein complexes that help cells connect to other cells and the extracellular matrix. Anchoring junctions are present on the lateral and basal surfaces of cells, providing strong and flexible connections. Focal adhesions are often formed due to cell interactions with the ECM substrata, which initiate signal transduction via kinase cascades and other mechanisms. Together, they provide stability and tissue integrity. There are three types of anchoring junctions:...
3.7K
Hedgehog Signaling Pathway02:33

Hedgehog Signaling Pathway

7.3K
The Hedgehog gene (Hh) was first discovered due to its control of the growth of disorganized, hair-like bristles phenotype in Drosophila, much like hedgehog spines. Hh plays a crucial role in the development of organs and the maintenance of homeostasis in both invertebrates and vertebrates. However, while Drosophila has only one Hh protein, mammals have multiple functional Hedgehog proteins - Sonic (Shh), Desert (Dhh), and Indian Hedgehog (Ihh). All of these homologous proteins have adapted to...
7.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A direct interaction of JAM-C with the tight junction scaffold protein ZO-2.

Scientific reports·2026
Same author

Uncovering the electrical synapse proteome in retinal neurons via in vivo proximity labeling.

eLife·2026
Same author

Modeling aging in a culture dish: towards the development of more sophisticated in vitro models of human skin aging.

Ageing research reviews·2026
Same author

Spectrin coordinates cell shape and signaling essential for epidermal differentiation.

The Journal of cell biology·2026
Same author

Identification of novel fibroblast subsets in diffuse cutaneous systemic sclerosis.

Annals of the rheumatic diseases·2025
Same author

Sticking to Membranes: Structure, Function, and Cellular Roles of the Annexin Family of Ca<sup>2+</sup>- and Membrane-Binding Proteins.

Cold Spring Harbor perspectives in biology·2025

Related Experiment Video

Updated: Jun 18, 2025

The C. elegans Intestine As a Model for Intercellular Lumen Morphogenesis and In Vivo Polarized Membrane Biogenesis at the Single-cell Level: Labeling by Antibody Staining, RNAi Loss-of-function Analy
12:15

The C. elegans Intestine As a Model for Intercellular Lumen Morphogenesis and In Vivo Polarized Membrane Biogenesis at the Single-cell Level: Labeling by Antibody Staining, RNAi Loss-of-function Analy

Published on: October 3, 2017

13.4K

RhoGDI1 regulates cell-cell junctions in polarized epithelial cells.

Nicolina Wibbe1, Tim Steinbacher1, Frederik Tellkamp2,3

  • 1Institute-Associated Research Group "Cell Adhesion and Cell Polarity", Institute of Medical Biochemistry, Zentrum für Molekularbiologie der Entzündung, University Münster, Münster, Germany.

Frontiers in Cell and Developmental Biology
|August 1, 2024
PubMed
Summary

Rho GDP dissociation inhibitor 1 (RhoGDI1) is crucial for epithelial cell junction formation and migration. Its depletion delays tight junction development and impairs cell migration responses.

Keywords:
ARHGDIAJAM-ARhoGDI1contact inhibition of locomotionepithelial barriertight junction

More Related Videos

Analyzing the Function of Small GTPases by Microinjection of Plasmids into Polarized Epithelial Cells
09:38

Analyzing the Function of Small GTPases by Microinjection of Plasmids into Polarized Epithelial Cells

Published on: May 31, 2011

11.4K
Differentiation of Mouse Breast Epithelial HC11 and EpH4 Cells
09:32

Differentiation of Mouse Breast Epithelial HC11 and EpH4 Cells

Published on: February 27, 2020

8.8K

Related Experiment Videos

Last Updated: Jun 18, 2025

The C. elegans Intestine As a Model for Intercellular Lumen Morphogenesis and In Vivo Polarized Membrane Biogenesis at the Single-cell Level: Labeling by Antibody Staining, RNAi Loss-of-function Analy
12:15

The C. elegans Intestine As a Model for Intercellular Lumen Morphogenesis and In Vivo Polarized Membrane Biogenesis at the Single-cell Level: Labeling by Antibody Staining, RNAi Loss-of-function Analy

Published on: October 3, 2017

13.4K
Analyzing the Function of Small GTPases by Microinjection of Plasmids into Polarized Epithelial Cells
09:38

Analyzing the Function of Small GTPases by Microinjection of Plasmids into Polarized Epithelial Cells

Published on: May 31, 2011

11.4K
Differentiation of Mouse Breast Epithelial HC11 and EpH4 Cells
09:32

Differentiation of Mouse Breast Epithelial HC11 and EpH4 Cells

Published on: February 27, 2020

8.8K

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Epithelial Cell Biology

Background:

  • Cell-cell contact formation in polarized epithelial cells relies on Rho GTPases.
  • Rho guanine nucleotide exchange factors (GEFs) and Rho GTPase-activating proteins (GAPs) are known regulators at cell junctions.
  • The function of Rho GDP dissociation inhibitors (GDIs) in this process remains largely uncharacterized.

Purpose of the Study:

  • To investigate the role of RhoGDI1 (ARHGDIA) in the formation of cell-cell contacts in polarized epithelial cells.
  • To elucidate the involvement of RhoGDI1 in junction maturation and cell migration dynamics.

Main Methods:

  • Depletion of RhoGDI1 using specific techniques.
  • Analysis of cell-cell junction development and tight junction formation.
  • Assessment of cell migration behavior upon cell collision and collective migration.

Main Results:

  • RhoGDI1 depletion significantly delays the formation of linear cell-cell junctions and barrier-forming tight junctions.
  • Loss of RhoGDI1 impairs the cessation of cell migration following cell-cell collision.
  • RhoGDI1 depletion leads to an increased migration velocity in collectively migrating cells.
  • The cell adhesion receptor JAM-A facilitates RhoGDI1 recruitment to cell-cell contacts.

Conclusions:

  • RhoGDI1 plays a critical role in regulating cell-cell contact formation and junction maturation in epithelial cells.
  • RhoGDI1 influences the dynamic reorganization of cell-cell junctions and cell migration.
  • JAM-A-mediated recruitment of RhoGDI1 highlights its integration into cell adhesion pathways.