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RNA Interference

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...

RNA-seq

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases.
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while microarray-based...

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Updated: May 23, 2026

Simultaneous Mapping and Quantitation of Ribonucleotides in Human Mitochondrial DNA

Simultaneous Mapping and Quantitation of Ribonucleotides in Human Mitochondrial DNA

Published on: November 14, 2017

Guidelines for mitochondrial RNA analysis.

Amela Jusic^1,2, Zoi Erpapazoglou³, Louise Torp Dalgaard⁴

¹HAYA Therapeutics SA, Route De La Corniche 6, SuperLab Suisse - Batiment Serine, 1066 Epalinges, Switzerland.

Molecular Therapy. Nucleic Acids

|August 2, 2024

Summary

This summary is machine-generated.

Mitochondrial RNAs are crucial for cell communication and function, but studying them is challenging. Standardized methods are needed to improve research accuracy and clinical applications for diseases.

Keywords:

MT: Non-coding RNAs gene expression guidelines microRNAs mitochondria noncoding RNAs

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Last Updated: May 23, 2026

Simultaneous Mapping and Quantitation of Ribonucleotides in Human Mitochondrial DNA

Simultaneous Mapping and Quantitation of Ribonucleotides in Human Mitochondrial DNA

Published on: November 14, 2017

Discrimintion and Mapping of the Primary and Processed Transcripts in Maize Mitochondrion Using a Circular RT-PCR-based Strategy

Discrimintion and Mapping of the Primary and Processed Transcripts in Maize Mitochondrion Using a Circular RT-PCR-based Strategy

Published on: July 29, 2019

Genotyping Single Nucleotide Polymorphisms in the Mitochondrial Genome by Pyrosequencing

Genotyping Single Nucleotide Polymorphisms in the Mitochondrial Genome by Pyrosequencing

Published on: February 10, 2023

Area of Science:

Mitochondrial biology
Molecular genetics
Cellular signaling

Background:

Mitochondria are vital energy producers in mammalian cells, essential for metabolism and signaling.
Mitochondrial RNAs (mtRNAs), including microRNAs and long noncoding RNAs, are encoded by mitochondrial and nuclear genomes.
These mtRNAs mediate nucleus-mitochondria communication, impacting physiological and pathological states.

Purpose of the Study:

To address challenges in studying mitochondrial RNAs.
To summarize current techniques and identify sources of variability in mtRNA research.
To advocate for standardized protocols to enhance research reproducibility and clinical translation.

Main Methods:

Review and summarization of existing techniques for mitochondrial RNA analysis.
Identification of critical factors causing variability in experimental results.
Emphasis on the need for standardized protocols and guidelines.

Main Results:

Significant challenges exist in the deep functional characterization and expression profiling of mitochondrial RNAs.
Lack of standardized protocols leads to discrepancies in published research findings.
EU-CardioRNA and AtheroNET COST Action networks highlight key areas for improvement.

Conclusions:

Standardized methods are essential for accurate quantification and study of mitochondrial RNAs.
Adherence to guidelines will improve research output, reproducibility, and clinical translation potential.
Establishing best practices will advance the field of mitochondrial RNA research for disease treatment.