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Updated: Jul 18, 2026
Development and Validation of a Quantitative PCR Method for Equid Herpesvirus-2 Diagnostics in Respiratory Fluids
Published on: March 17, 2016
Molecular testing for equine herpesviruses 1 (EHV-1) in healthy postpartum broodmares
Luis G Arroyo1, Diego E Gomez1, Alison Moore1
1Departments of Clinical Studies (Arroyo, Gomez) and Pathobiology (Papapetrou, Lillie), Ontario Veterinary College, University of Guelph, 50 Stone Road East, Guelph, Ontario N1G 2W1; Ontario Ministry of Agriculture, Food and Rural Affairs, 5th Floor NW, 1 Stone Road West, Guelph, Ontario N1G 4Y2 (Moore).
Objective:
Our objective was to determine whether equine herpesviruses 1 (EHV-1) viral nucleic acids could be detected immediately after foaling from nasal and vaginal swabs, whole blood, and placental tissue of healthy mares.
Animals Procedure And Results:
Nasal and vaginal swabs, EDTA blood, and placental tissue (296 samples) were collected from 74 clinically healthy postpartum broodmares within 24 h after giving birth to live, clinically healthy foals. All samples were tested (PCR) for nucleic acids of neuropathogenic and non-neuropathogenic strains of EHV-1, and all were negative.
Conclusion And Clinical Relevance:
As EHV-1 was not detected in the immediate postpartum period in healthy mares with uncomplicated foaling, we inferred that EHV-1-positive samples from aborting mares and/or EHV-1 detection in fetal membranes indicate EHV-1-associated abortion.
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