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In Situ Colorimetric LAMP Based on One-Step Modified Filter Paper to Screen Human Papillomavirus (HPV)16/18 from
Tingting Jiao1, Peng Sun2, Shuang Tian1
1Reproductive Medical Center, Department of Obstetrics and Gynecology, Seventh Medical Center of PLA General Hospital, Beijing 100700, China.
Langmuir : the ACS Journal of Surfaces and Colloids
|August 2, 2024
Summary
We developed a rapid HPV screening method using chitosan-modified filter paper and colorimetric LAMP for early cervical cancer detection. This technique accurately identifies HPV 16/18, aiding epidemiological control and on-site testing.
Area of Science:
- Molecular Biology
- Biotechnology
- Oncology
Background:
- Cervical cancer is a leading malignancy in women, necessitating effective early detection strategies.
- Current screening methods can be improved for speed and accessibility, especially in resource-limited settings.
- Human Papillomavirus (HPV) is a primary cause of cervical cancer, making HPV detection crucial for prevention.
Purpose of the Study:
- To develop a rapid, reliable, and accessible HPV screening method for early detection of cervical cancer.
- To integrate nucleic acid enrichment and visual detection into a single, user-friendly platform.
- To assess the method's performance using clinical samples and its potential for on-site application.
Main Methods:
- Developed a chitosan-modified filter paper for high-efficiency nucleic acid enrichment.
- Utilized pH-sensitive colorimetric Loop-Mediated Isothermal Amplification (LAMP) for rapid visual detection.
- Integrated these components into a disposable chip for simultaneous DNA concentration, amplification, and detection without prior nucleic acid extraction.
Main Results:
- The developed method achieved 100% concordance with clinical diagnosis for HPV 16/18 detection in endometrial samples.
- The system demonstrated high sensitivity, accurately identifying HPV 16/18 even in samples diluted 100-fold.
- The filter paper effectively enriched cells and mitigated issues from acidic samples, enabling direct amplification.
Conclusions:
- This novel HPV screening technique offers a rapid, sensitive, and reliable approach for early cervical cancer detection.
- The integrated system simplifies the testing process, making it suitable for routine on-site screening and near-patient diagnostics.
- The method holds significant potential for improving HPV-induced cancer prevention and epidemiological control strategies globally.
