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Ad-VT causes ovarian cancer A2780 cell death via mitochondrial apoptosis and autophagy pathways

Yingli Cui1, Xin Chen2, Wenjie Li3

  • 1Department of Gynecologic Oncology, First Hospital of Jilin University, Changchun 130021, PR China.

Translational Oncology
|August 2, 2024
PubMed
Abstract

Insights

Recombinant adenovirus Ad-apoptin-hTERTp-E1a (Ad-VT) effectively inhibits ovarian cancer cells by inducing apoptosis and autophagy. This oncolytic virus shows significant antitumor effects in vivo, offering a promising therapeutic strategy.

Area of Science:

  • Oncolytic virology
  • Cancer biology
  • Molecular oncology

Background:

  • Recombinant adenovirus Ad-apoptin-hTERTp-E1a (Ad-VT) exhibits bi-specific oncolytic activity.
  • The precise mechanisms of Ad-VT-induced tumor cell death remain incompletely understood.
  • Investigating the interplay between apoptosis and autophagy in Ad-VT's action is crucial.

Purpose of the Study:

  • To elucidate the apoptosis and autophagy mechanisms induced by Ad-VT in ovarian cancer cells.
  • To explore the interaction between autophagy and apoptosis pathways during Ad-VT treatment.
  • To evaluate the therapeutic potential of Ad-VT against ovarian cancer.

Main Methods:

  • In vitro assays (Crystal Violet staining, CCK-8) assessed Ad-VT's effect on ovarian cancer cells.
  • In vivo studies utilized a tumor-bearing nude mouse model to evaluate antitumor efficacy.
  • Flow cytometry and fluorescence staining (LC3) analyzed apoptosis and autophagy levels.

Main Results:

  • Ad-VT demonstrated significant inhibition of ovarian cancer A2780 cells with minimal impact on normal cells.
  • In vivo experiments confirmed Ad-VT's tumor growth inhibition and survival extension in mice.
  • Ad-VT induced significant apoptosis via the endogenous pathway and increased protective autophagy in cancer cells.

Conclusions:

  • Ad-VT effectively targets and inhibits ovarian cancer A2780 cells.
  • The virus promotes both apoptosis and autophagy, contributing to its oncolytic effect.
  • Ad-VT represents a potential therapeutic agent for ovarian cancer treatment.
Keywords:
ApoptinApoptosisAutophagyOvarian cancerRecombinant adenovirus

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