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Biochemical Titration of Glycogen In vitro
Published on: November 24, 2013
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Bioluminescent Assay for the Quantification of Cellular Glycogen Levels
Donna Leippe1, Rebeca Choy2, Gediminas Vidugiris1
1Research and Development, Promega Corporation, Madison, Wisconsin 53711, United States.
ACS Omega
|August 5, 2024
Summary
A new bioluminescent assay accurately measures cellular glycogen, aiding the development of glycogen synthase inhibitors for treating Pompe disease and other glycogen storage disorders.
Area of Science:
- Biochemistry
- Cell Biology
- Pharmacology
Background:
- Glycogen, a glucose polymer, is vital for energy storage and glucose homeostasis.
- Dysregulation of glycogen metabolism causes diseases like Pompe disease (Glycogen Storage Disease II).
- Pompe disease results in muscle atrophy and respiratory issues due to excessive glycogen accumulation.
Purpose of the Study:
- To develop a high-throughput assay for measuring cellular glycogen.
- To facilitate the study and screening of glycogen synthase inhibitors.
- To support therapeutic development for Pompe disease.
Main Methods:
- Automated bioluminescent glycogen detection assay.
- Quantification of glycogen in cultured cells grown in 384-well plates.
- Treatment of cells with glycogen synthase 1 inhibitors.
Main Results:
- The assay successfully quantified glycogen levels in cells.
- Reduced glycogen stores were observed in cells treated with inhibitors.
- The assay demonstrated utility for drug screening and research.
Conclusions:
- The developed bioluminescent assay is effective for measuring cellular glycogen.
- This assay supports the development of glycogen synthase inhibitors for Pompe disease.
- The method advances research in glycogen metabolism and therapeutic strategies.
