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Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...

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Multiplex PCR Assay for Typing of Staphylococcal Cassette Chromosome Mec Types I to V in Methicillin-resistant Staphylococcus aureus

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A novel PCR-based genotyping method for Proteus mirabilis - Intergenic region polymorphism analysis.

Nianqing Kong¹, Yilin Hu², Chenglu Lan²

¹College of Food Science, Guangdong Pharmaceutical University, Zhongshan, Guangdong 528458, China; Nanfang Hospital Baiyun Branch, Southern Medical University, Guangzhou, Guangdong 510600, China.

Journal of Microbiological Methods

|August 5, 2024

Summary

Intergenic region polymorphism analysis (IRPA) effectively differentiates Proteus mirabilis strains from food sources. This rapid genotyping method shows higher discriminatory power than ERIC-PCR for P. mirabilis.

Keywords:

Antimicrobials resistance ERIC-PCR Genotyping IRPA Proteus mirabilis

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Genotyping of Staphylococcus aureus by Ribosomal Spacer PCR RS-PCR

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Published on: November 4, 2016

Area of Science:

Microbiology
Molecular Biology
Food Safety

Background:

Proteus mirabilis is a significant cause of food poisoning from meat products and an opportunistic human pathogen.
Accurate strain differentiation is crucial for tracking foodborne illnesses and understanding P. mirabilis epidemiology.

Purpose of the Study:

To develop and evaluate the Intergenic Region Polymorphism Analysis (IRPA) method for differentiating P. mirabilis isolates.
To compare the discriminatory power of IRPA with ERIC-PCR for P. mirabilis genotyping.

Main Methods:

Developed an IRPA typing scheme to amplify polymorphic fragments in intergenic regions (IGRs) of P. mirabilis.
Genotyped 75 P. mirabilis isolates from poultry and pork samples using IRPA and ERIC-PCR.
Assessed antibiotic resistance profiles of the isolates.

Main Results:

IRPA demonstrated a higher Simpson's index of diversity (0.999) compared to ERIC-PCR (0.923), indicating superior discriminatory power.
IRPA resolved isolates within the same ERIC cluster into separate clusters, highlighting its high resolution.
High resistance rates were observed for tetracycline, doxycycline, chloramphenicol, streptomycin, spectinomycin, trimethoprim, trimethoprim-sulfamethoxazole, and erythromycin.

Conclusions:

IRPA is a rapid, simple, reproducible, and economical method for P. mirabilis genotyping.
IRPA offers enhanced discriminatory power for differentiating P. mirabilis strains compared to ERIC-PCR.
The study provides valuable insights into the genetic diversity and antibiotic resistance of P. mirabilis in meat products.