Effects of Non-Thermal Dielectric Barrier Discharge Plasma on Human Fibroblasts: A Narrative Review

Objectives: Fibroblasts are among the most critical connective tissue cells in almost all tissues and organs. Enhancement of fibroblast differentiation, proliferation, and morphogenesis is of paramount importance in tissue regeneration and wound healing. The non-thermal dielectric barrier discharge (DBD) plasma technology has recently gained interest due to its extensive applications and multiple biological effects. This review article outlines the applications of DBD plasma in dentistry, and its biological effects on human fibroblasts. Materials and Methods: Relevant keywords were searched in PubMed, Ovid, and Google Scholar online databases. The search strategy resulted in selection of 7 studies according to the eligibility criteria. Results: Most studies reported increased cell proliferation and viability after the application of DBD plasma. Four studies that focused on the development of adhesion-related appendages examined the morphology of fibroblast cells, including the creation of vinculin, protrusion, and actin cytoskeleton. Expression of cyclin D1/P27 genes and genes associated with adhesion and cell attachments was also reported in two studies. Conclusion: This narrative review discussed the effects of DBD plasma technology on proliferation and behavior of human fibroblasts, and reviewed the available articles in this regard. More in vivo studies are required to understand the exact effects of this emerging technology on human mesenchymal tissues.