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Overview of Exosomes

Overview of Exosomes

Exosomes are stable, lipid bilayer-enclosed vesicles capable of crossing biological barriers. They can carry a wide range of molecules required for intercellular communication. Once exosomes are released from the cell where they originated, they enter a recipient cell through various pathways such as fusion, receptor-mediated endocytosis, macropinocytosis, and phagocytosis.
Stahl et al. discovered exosomes in 1983, but the exosomes were initially considered waste products released from the...

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Updated: Jun 17, 2025

Isolation and Characterization of RNA-Containing Exosomes

Isolation and Characterization of RNA-Containing Exosomes

Published on: January 9, 2012

Exosomes Isolation, Purification, and Characterization.

Prabhat Ranjan¹, Suresh Kumar Verma²

¹Department of Medicine, Division of Cardiovascular Disease, The University of Alabama at Birmingham, Birmingham, AL, USA.

Methods in Molecular Biology (Clifton, N.J.)

|August 6, 2024

Summary

This summary is machine-generated.

This chapter details exosome isolation and characterization methods. It addresses challenges in obtaining high-yield, quality exosomes for expanding biomedical applications.

Keywords:

Bone marrow-progenitor cells Endothelial cells Exosomes Fibroblasts Sucrose density gradient Ultracentrifugation

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Last Updated: Jun 17, 2025

Isolation and Characterization of RNA-Containing Exosomes

Isolation and Characterization of RNA-Containing Exosomes

Published on: January 9, 2012

Isolation and Profiling of MicroRNA-containing Exosomes from Human Bile

Isolation and Profiling of MicroRNA-containing Exosomes from Human Bile

Published on: June 13, 2016

Isolation and Characterization of Exosomes from Skeletal Muscle Fibroblasts

Isolation and Characterization of Exosomes from Skeletal Muscle Fibroblasts

Published on: May 16, 2020

Area of Science:

Cell Biology
Nanotechnology
Biochemistry

Background:

Exosomes are nanovesicles secreted by cells, crucial in intercellular communication.
Their molecular cargo influences cellular functions and disease states.
Exosome research is advancing, with growing interest in biomedical uses.

Purpose of the Study:

To describe protocols for exosome isolation and characterization.
To present alternative purification methods for exosomes.
To address challenges in achieving high yield and quality of isolated exosomes.

Main Methods:

Detailed protocols for exosome isolation.
Methods for exosome characterization.
Discussion of alternative purification techniques.

Main Results:

Established protocols for exosome isolation and characterization.
Exploration of alternative purification strategies.
Guidance on overcoming yield and quality challenges.

Conclusions:

Effective isolation and characterization are key to exosome utility.
Alternative methods offer flexibility in exosome purification.
Optimized protocols are essential for advancing exosome-based biomedical applications.