Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Stem Cell Therapy for Tissue Regeneration01:21

Stem Cell Therapy for Tissue Regeneration

Stem cell therapy is a method used in regenerative medicine to repair and restore function to damaged tissues and organs. Stem cells have the potential to proliferate and differentiate into various tissue types, making them ideal candidates for tissue regeneration. For example, hematopoietic stem cell transplants are commonly used in blood cancer treatment to replenish damaged bone marrow and restore healthy blood cells.
Types of Stem Cells used in Stem Cell Therapy
The two main cell types that...
Bone Marrow Sampling and Transplants01:22

Bone Marrow Sampling and Transplants

Bone marrow transplant is a potential cure for several diseases, including cancer and specific genetic disorders. Notably, this procedure is applicable for patients suffering from aplastic anemia, certain types of leukemia, severe combined immunodeficiency disease (SCID), Hodgkin's disease, non-Hodgkin's lymphoma, multiple myeloma, thalassemia, sickle-cell disease, and certain cancers.
The transplant begins with high doses of chemotherapy and radiation treatment, which aim to destroy the...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Nectin-4 expression in clear cell renal cell carcinoma.

Scientific reports·2026
Same author

Corrigendum to 'EAU-ESMO Consensus Statements on the Management of Advanced and Variant Bladder Cancer-An International Collaborative Multistakeholder Effort Under the Auspices of the EAU-ESMO Guidelines Committees' [European Urology 77 (2020) 223-250].

European urology·2020
Same author

An evaluation of avelumab for the treatment of genitourinary tumors.

Expert opinion on biological therapy·2020
Same author

Impact of Histopathological Prostate Inflammation on Urine-Based Prostate Cancer Prediction Using the Prostate Cancer Gene 3 Score.

Urologia internationalis·2020
Same author

European Association of Urology Guidelines Office Rapid Reaction Group: An Organisation-wide Collaborative Effort to Adapt the European Association of Urology Guidelines Recommendations to the Coronavirus Disease 2019 Era.

European urology·2020
Same author

Effect of Enzalutamide plus Androgen Deprivation Therapy on Health-related Quality of Life in Patients with Metastatic Hormone-sensitive Prostate Cancer: An Analysis of the ARCHES Randomised, Placebo-controlled, Phase 3 Study.

European urology·2020

Related Experiment Video

Updated: Jun 30, 2026

An Orthotopic Bladder Cancer Model for Gene Delivery Studies
07:48

An Orthotopic Bladder Cancer Model for Gene Delivery Studies

Published on: December 1, 2013

12.5K

Patient-derived bladder cancer organoids show stable transcript expression along cultivation.

Philipp Vollmer1, Bastian Amend2, Niklas Harland2

  • 1Center for Medical Research, University of Tuebingen, 72074, Tübingen, Germany.

World Journal of Urology
|August 7, 2024
PubMed
Summary

Patient-derived bladder cancer organoids (BCOs) maintain stable transcriptomes across passages, supporting their use in personalized medicine and drug screening. Earlier passages are ideal for rapid treatment counseling, while higher passages suit cell mass-dependent applications.

Keywords:
Bladder cancerBladder cancer organoidsDisease modelingPersonalized medicineTranscriptomics

More Related Videos

Culture, Manipulation, and Orthotopic Transplantation of Mouse Bladder Tumor Organoids
08:43

Culture, Manipulation, and Orthotopic Transplantation of Mouse Bladder Tumor Organoids

Published on: January 31, 2020

9.7K
Culture of Bladder Cancer Organoids as Precision Medicine Tools
08:39

Culture of Bladder Cancer Organoids as Precision Medicine Tools

Published on: December 28, 2021

4.6K

Related Experiment Videos

Last Updated: Jun 30, 2026

An Orthotopic Bladder Cancer Model for Gene Delivery Studies
07:48

An Orthotopic Bladder Cancer Model for Gene Delivery Studies

Published on: December 1, 2013

12.5K
Culture, Manipulation, and Orthotopic Transplantation of Mouse Bladder Tumor Organoids
08:43

Culture, Manipulation, and Orthotopic Transplantation of Mouse Bladder Tumor Organoids

Published on: January 31, 2020

9.7K
Culture of Bladder Cancer Organoids as Precision Medicine Tools
08:39

Culture of Bladder Cancer Organoids as Precision Medicine Tools

Published on: December 28, 2021

4.6K

Area of Science:

  • Oncology
  • Genomics
  • Biotechnology

Background:

  • Bladder cancer (BC) is a common malignancy with frequent recurrences.
  • Patient-derived bladder cancer organoids (BCOs) offer potential for disease modeling and personalized treatment screening.
  • Understanding BCO transcriptomic stability during cultivation is crucial for their application.

Purpose of the Study:

  • To investigate transcriptomic plasticity in bladder cancer organoids (BCOs) over different cultivation periods.
  • To determine optimal timeframes for utilizing BCOs in research and clinical applications.
  • To assess the stability of gene expression profiles in BCOs throughout expansion.

Main Methods:

  • Tumor samples from three bladder cancer patients were used to establish BCOs.
  • Differential gene expression and quantitative real-time PCR (qRT-PCR) analyzed RNA expression at various passages.
  • Transcriptomic profiles were compared across early (1-4), mid (5-9), and late (>9) passages.

Main Results:

  • BCO lines exhibited consistent transcriptomic profiles without significant alterations across passages.
  • Key cancer-related genes (e.g., TP53, PIK3CA, BRCA1) showed stable expression levels during cultivation.
  • No substantial transcriptomic changes were observed throughout the BCO expansion process.

Conclusions:

  • The stable transcriptome of BCOs supports their use in personalized medicine and efficient drug screening.
  • Earlier BCO passages are recommended for accelerated patient treatment counseling.
  • Higher BCO passages are suitable for applications requiring larger cell quantities, such as medical device development.