Programming of a Portable Digital Monitoring System-Integrated DNA Aptamer Reversely Regulated Oxidase-Like Nanozyme...

Area of Science:

• Environmental Science
• Analytical Chemistry
• Materials Science

Background:

• Per- and polyfluoroalkyl substances (PFAS) pose environmental risks, necessitating efficient atmospheric monitoring.
• Accurate detection of specific PFAS, like perfluorooctanoic acid (PFOA), is crucial for pollution control and early warning systems.

Purpose of the Study:

• To develop a portable, intelligent digital monitoring device for the real-time, onsite analysis of atmospheric PFOA.
• To engineer a novel nanozyme-based sensing mechanism for enhanced PFOA detection.

Main Methods:

• A PtCoNPs@g-C3N4 nanozyme was surface-modified with a PFOA-specific DNA aptamer, creating Apt-PtCoNPs@g-C3N4.
• The system utilizes the PFOA-activated oxidase-like activity of the nanozyme to oxidize o-phenylenediamine (OPD) into a fluorescent product (DAP).
• A hydrogel-based kit integrated into a portable device captured and processed dual-modality (colorimetric and fluorescence) signals for PFOA quantification.

Main Results:

• The Apt-PtCoNPs@g-C3N4 nanozyme exhibited PFOA-triggered restoration of oxidase-like activity.
• A dual-modality colorimetric and fluorescence response was observed upon PFOA interaction.
• The portable device successfully performed onsite, real-time dynamic analysis of atmospheric PFOA.

Conclusions:

• The developed portable device offers a reliable and intelligent solution for atmospheric PFOA monitoring.
• This approach provides a foundation for designing similar portable devices for detecting other atmospheric contaminants.
• The study enhances capabilities for environmental pollution traceability and early warning systems.