Decoding of the isotopic fingerprint of tequila 100% agave silver class and image analysis to evaluate differences...

This communication shows the decoding of Isotopic Fingerprint of Tequila 100% agave silver class (IF_{Tequila100% agave}) in three areas corresponding to isotopic variations due to: plant used as raw material, fermentation and distillation process, and hydrolysis process. Isotopic tracers that make them up correspond to the δ¹³C_{VPDB ethanol}-δ¹³C_{VPDB ethyl acetate}-δ¹³C_{VPDB isoamyl alcohol}, δ¹³C_{VPDB ethyl acetate}-δ¹³C_{VPDB isoamyl alcohol}-δ¹³C_{VPDB n-propanol} and δ¹³C_{VPDB ethyl acetate}-δ¹³C_{VPDB n-propanol}-δ¹³C_{VPDB methanol}, respectively. Once the IF_{Tequila100%agave} has been decoded, an image comparison was performed against isotopic fingerprints of spirits (Tequila, Bacanora, Raicilla, Sotol, and Mezcal). Results show that it is possible classifies 100% of samples analyzed. Likewise, from decoding it is possible to determine the critical process stage to determine variations with respect to the IF_{Tequila100%agave}. The chemometric analysis developed corresponds to an auxiliary analytical tool useful for the inspection processes currently carried out by the authorities to determine the authenticity of the beverage.