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Updated: Jun 17, 2025

In Vitro and In Vivo Detection of Mitophagy in Human Cells, C. Elegans, and Mice

In Vitro and In Vivo Detection of Mitophagy in Human Cells, C. Elegans, and Mice

Published on: November 22, 2017

Assays to Study Mitophagy in Plants.

Chengyang Li^1,2, Juncai Ma³, Tian Zhen^1,2

¹National Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crops, College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan, China.

Methods in Molecular Biology (Clifton, N.J.)

|August 8, 2024

Summary

This summary is machine-generated.

This study presents methods for investigating mitophagy, a crucial process for plant development and stress response. These techniques aid in identifying new mitophagy regulators and understanding their roles in plant mitochondrial functions.

Keywords:

Arabidopsis thaliana De-etiolation Mitochondria Mitophagy Nicotiana benthamiana

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Published on: November 22, 2017

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Published on: September 15, 2023

Area of Science:

Plant Biology
Cell Biology
Molecular Biology

Background:

Mitophagy is essential for eukaryotic cell function, including plant development and stress adaptation.
Plant-specific mitophagy regulators, like Friendly (FMT) and TraB family proteins (TRB), are emerging areas of research.

Purpose of the Study:

To describe methodologies for studying mitophagy in plants.
To facilitate the identification and functional characterization of novel mitophagy regulators in plants.

Main Methods:

Utilizing conventional microscopy and loss-of-function mutants.
Employing transgenic mitochondrial marker lines with subsequent image analysis.
Applying chemical inhibitor treatments and analyzing plant phenotypes.

Main Results:

The described methods provide a framework for studying plant mitophagy.
These approaches enable the identification of potential new mitophagy regulators.
The methods aid in elucidating the functions of these regulators in mitochondrial activities.

Conclusions:

The presented combined methodologies offer a robust approach to plant mitophagy research.
This work supports the ongoing discovery and functional analysis of plant-specific mitophagy regulators.
Understanding mitophagy is key to comprehending plant mitochondrial dynamics and stress responses.