Acid-induced hydrogels of edible Chlorella pyrenoidosa protein with composite biopolymers network

This study aimed to prepare Glucono-δ-lactone (GDL)-induced Chlorella pyrenoidosa protein (CPP) hydrogel and further investigate the effect of polysaccharides on the mechanical properties and stability enhancement of the composite hydrogels. Polysaccharides composed of different ratios of low acyl gellan gum (GE) and guar gum (GU) imparted dense honeycomb-like networks and adjustable textural properties to the composite hydrogels induced by CaCl₂. In particular, the hardness of hydrogels increased significantly from 14 to 833 g. Scanning electron microscopy results revealed that CPP-GE/GU composite hydrogels had better stable spatial porous structures. Moreover, fourier transform infrared spectroscopy (FTIR) indicated hydrogen bonding interaction between CPP and GE/GU. The composite network showed improved viscoelasticity, increased thermal stability, and self-healing ability of hydrogels. The composite hydrogels also showed high water holding (89-98%) and swelling (747-862%) properties compared to the pure CPP hydrogel. These findings further expand CPP hydrogel products and broaden application in plant protein-based food.