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Electron Microscope Tomography and Single-particle Reconstruction

Electron Microscope Tomography and Single-particle Reconstruction

Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...

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Related Experiment Video

Updated: Jun 17, 2025

Using Tomoauto: A Protocol for High-throughput Automated Cryo-electron Tomography

Using Tomoauto: A Protocol for High-throughput Automated Cryo-electron Tomography

Published on: January 30, 2016

Smart parallel automated cryo-electron tomography.

Fabian Eisenstein^1,2, Yoshiyuki Fukuda³, Radostin Danev⁴

¹Graduate School of Medicine, University of Tokyo, Tokyo, Japan.

|August 8, 2024

Summary

This summary is machine-generated.

We developed SPACEtomo, a machine learning workflow that fully automates cryo-electron tomography. This automation is key for high-resolution macromolecule structure determination in cells.

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Cryo-Electron Tomography Remote Data Collection and Subtomogram Averaging

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User-friendly, High-throughput, and Fully Automated Data Acquisition Software for Single-particle Cryo-electron Microscopy

User-friendly, High-throughput, and Fully Automated Data Acquisition Software for Single-particle Cryo-electron Microscopy

Published on: July 29, 2021

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Last Updated: Jun 17, 2025

Using Tomoauto: A Protocol for High-throughput Automated Cryo-electron Tomography

Using Tomoauto: A Protocol for High-throughput Automated Cryo-electron Tomography

Published on: January 30, 2016

Cryo-Electron Tomography Remote Data Collection and Subtomogram Averaging

Cryo-Electron Tomography Remote Data Collection and Subtomogram Averaging

Published on: July 12, 2022

User-friendly, High-throughput, and Fully Automated Data Acquisition Software for Single-particle Cryo-electron Microscopy

User-friendly, High-throughput, and Fully Automated Data Acquisition Software for Single-particle Cryo-electron Microscopy

Published on: July 29, 2021

Area of Science:

Structural Biology
Biophysics
Cell Biology

Background:

In situ cryo-electron tomography (cryo-ET) allows visualization of macromolecules within their native cellular environment.
Recent advancements in hardware and software have increased sample accessibility for cryo-ET.
Current cryo-ET data collection is labor-intensive, requiring expert operators and significant microscope time for target selection and tilt series acquisition.

Purpose of the Study:

To develop a fully automated workflow for cryo-electron tomography.
To eliminate the need for human intervention in cryo-ET data collection.
To enable high-throughput acquisition of statistically relevant datasets for structural analysis.

Main Methods:

Developed SPACEtomo, a novel workflow integrating machine learning.
Implemented automated lamella detection and biological feature segmentation.
Enabled automated target selection and parallel tilt series acquisition.

Main Results:

SPACEtomo fully automates the entire cryo-electron tomography process.
The workflow requires no human intervention.
Facilitates high-throughput data collection for structural studies.

Conclusions:

SPACEtomo significantly advances the automation of cryo-electron tomography.
This automation is crucial for obtaining statistically relevant datasets.
Enables high-resolution structure determination of macromolecules in their native cellular context.