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Computed Tomography01:10

Computed Tomography

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Tomography refers to imaging by sections. Computed tomography (CT) is a non-invasive imaging technique that uses computers to analyze several cross-sectional X-rays to reveal minute details about structures in the body.
The technique was invented in the 1970s and is based on the principle that as X-rays pass through the body, they are absorbed or reflected at different levels. In the technique, a patient lies on a motorized platform while a computerized axial tomography (CAT) scanner rotates...
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Electron Microscope Tomography and Single-particle Reconstruction01:07

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Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
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Imaging Studies I: CT and MRI01:14

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Introduction: MRI and CT scans are crucial advancements in medical imaging techniques, playing a vital role in diagnosing conditions related to the gastrointestinal (GI) system. Each scan serves distinct purposes, targets specific areas, and requires unique nursing duties.
Description of the Procedures
Computed Tomography (CT) scan:
Computed Tomography (CT) scans use X-ray technology to generate detailed images of bones, organs, and tissues. During the scan, the patient lies on a moving table...
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Positron Emission Tomography01:29

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Positron emission tomography (PET) is a medical imaging technique involving radiopharmaceuticals — substances that emit short-lived radiation. Although the first PET scanner was introduced in 1961, it took 15 more years before radiopharmaceuticals were combined with the technique and revolutionized its potential.
One of the main requirements of a PET scan is a positron-emitting radioisotope, which is produced in a cyclotron and then attached to a substance used by the part of the body...
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Brain Imaging01:14

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Brain imaging technologies provide critical insights into both the structure and function of the human brain, enabling medical professionals and researchers to diagnose, study, and treat neurological disorders or psychiatric disorders more effectively.
These technologies include computerized axial tomography (CAT or CT scans), positron-emission tomography (PET scans),  magnetic resonance imaging (MRI),  functional magnetic resonance imaging (fMRI), and Transcranial Magnetic...
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X-ray Imaging01:24

X-ray Imaging

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German physicist Wilhelm Röntgen (1845–1923) was experimenting with electrical current when he discovered that a mysterious and invisible "ray" would pass through his flesh but leave an outline of his bones on a screen coated with a metal compound. In 1895, Röntgen made the first durable record of the internal parts of a living human: an "X-ray" image (as it came to be called) of his wife’s hand. Scientists worldwide quickly began their own experiments with...
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Related Experiment Video

Updated: Jun 17, 2025

Array Tomography Workflow for the Targeted Acquisition of Volume Information using Scanning Electron Microscopy
09:47

Array Tomography Workflow for the Targeted Acquisition of Volume Information using Scanning Electron Microscopy

Published on: July 15, 2021

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Array tomography: trails to discovery.

Kristina D Micheva1, Jemima J Burden2, Martina Schifferer3,4

  • 1Department of Neurosurgery, Stanford University, Stanford, CA, 94305, USA.

Methods in Microscopy
|August 9, 2024
PubMed
Summary
This summary is machine-generated.

Array tomography (AT) enables detailed 3D biological structure analysis using serial sectioning and advanced microscopy. Novel developments simplify AT, making this powerful technique more accessible for researchers.

Keywords:
ATUMarray tomographylight microscopyserial sectioningultramicrotomyvolume electron microscopy

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Area of Science:

  • Biological imaging
  • Microscopy techniques
  • Structural biology

Background:

  • Tissue slicing is crucial for studying biological structures.
  • Volume electron microscopy (vEM) methods like array tomography (AT) offer advanced 3D analysis.
  • AT combines serial ultramicrotomy, section imaging, and data re-assembly.

Purpose of the Study:

  • To highlight advancements in array tomography (AT) for biological research.
  • To address the underrepresentation of AT by detailing solutions and overcoming constraints.
  • To guide researchers in selecting appropriate vEM techniques and exploring AT's potential.

Main Methods:

  • Serial ultramicrotomy for ultrathin sectioning.
  • Multi-modal imaging (light and scanning electron microscopy).
  • Image re-assembly and analysis for 3D volume reconstruction.

Main Results:

  • AT provides long-term sample preservation and multi-scale imaging compatibility.
  • Serial sectioning enhances axial resolution and facilitates molecular labeling for correlative microscopy.
  • New developments in sectioning and image analysis streamline the AT pipeline.

Conclusions:

  • Array tomography offers significant advantages for multi-scale and multi-modal biological imaging.
  • Overcoming perceived difficulties through new developments and training can increase AT adoption.
  • AT has vast unexplored potential across diverse biological fields, aiding structural and molecular insights.