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Updated: Jun 17, 2025
Pan-lyssavirus Real Time RT-PCR for Rabies Diagnosis
Published on: July 10, 2019
Real-time PCR assay to detect the novel Clade Ib monkeypox virus, September 2023 to May 2024
Leonard Schuele1,2, Leandre Murhula Masirika2,3,4,5, Jean Claude Udahemuka2,6,7
1Department of Viroscience, Erasmus University Medical Center, Rotterdam, the Netherlands.
Abstract:
Monkeypox virus (MPXV) is an emerging zoonotic pathogen with complex epidemiology necessitating rapid diagnosis and distinguishing between clades and subclades. The emerging Clade Ib lacks the genomic region used in the Clade I-specific assay from the Centers for Disease Control and Prevention. We report an MPXV real-time PCR to specifically detect Clade Ib. The assay demonstrated proficient sensitivity and specificity in 92 samples and can be included along other TaqMan-based assays to detect MPXV and distinguish between clades and subclades.
