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The purification of urease from Aspergillus nidulans
The International Journal of Biochemistry
|January 1, 1985
Abstract:
A purification procedure is described for Aspergillus urease, the most important step being affinity chromatography on hydroxyurea Sepharose. The enzyme exists as a single active species of Mr 240,000. The pure enzyme has an activity of 670 mumol urea hydrolysed/min, has a Km of 10(-3) M, an optimum pH of 8.5 and a sub-unit Mr of 40,000.