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A Ratiometric Fluorescence Detection Method for Berberine Using Triplex-Containing DNA-Templated Silver Nanoclusters.

Ming Zhu¹, Mingyang Sun¹, Juntong Liu¹

¹School of Pharmacy, Changchun University of Chinese Medicine, 1035 Boshuo Road, Changchun 130117, China.

Molecules (Basel, Switzerland)

|August 10, 2024

Summary

A new fluorescent method accurately detects berberine (BBR) using DNA-templated silver nanoclusters. This sensitive technique quantifies BBR in real samples, aiding pharmacological research and clinical use.

Keywords:

DNA-AgNCs berberine ratiometric fluorescence triplex

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Area of Science:

Analytical Chemistry
Biochemistry
Nanotechnology

Background:

Berberine (BBR), a natural isoquinoline alkaloid, possesses diverse pharmacological activities and is used in disease treatment.
Accurate quantification of BBR is crucial for pharmacological research and clinical applications.

Purpose of the Study:

To develop a sensitive and selective fluorescent detection method for Berberine (BBR).
To utilize the specific interaction between BBR and triplex DNA for quantitative analysis.

Main Methods:

A fluorescent detection method was established using DNA-templated silver nanoclusters (DNA-AgNCs).
The method leverages the fluorescence quenching of DNA-AgNCs upon binding with BBR to form a BBR-triplex complex.
Ratiometric fluorescence signals were measured to correlate with BBR concentration.

Main Results:

The method demonstrated a linear relationship between the ratiometric fluorescence signal and BBR concentration from 10 nM to 1000 nM.
A low detection limit of 10 nM was achieved.
The developed method showed excellent sensitivity and selectivity for BBR detection.

Conclusions:

A novel, sensitive, and selective fluorescent method for Berberine (BBR) detection was successfully developed.
The method, based on DNA-AgNCs and BBR-triplex interaction, is suitable for quantifying BBR in real samples.
This technique offers a valuable tool for pharmacological studies and clinical applications of Berberine.