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Transcriptome Profiling of Mouse Embryonic Fibroblast Spontaneous Immortalization: A Comparative Analysis
Jocshan Loaiza-Moss1, Ursula Braun1, Michael Leitges1
1Division of Biomedical Sciences, Faculty of Medicine, Memorial University of Newfoundland, 300 Prince Philip Drive, St. Johns, NL A1B 3V6, Canada.
International Journal of Molecular Sciences
|August 10, 2024
Summary
Spontaneous cell immortalization involves significant transcriptome changes, impacting proliferation and immune response. This study identifies key genes and proposes a regulatory network model for immortalized mouse embryonic fibroblasts (MEFs).
Area of Science:
- Molecular Biology
- Genomics
- Cancer Research
Background:
- Cell immortalization is a critical step in cancer development.
- Global transcriptome changes during spontaneous mouse embryonic fibroblast (MEF) immortalization are not well understood.
Purpose of the Study:
- To characterize poly-A RNA transcriptome alterations during spontaneous MEF immortalization.
- To identify differentially expressed genes (DEGs) and potential hub genes involved in this process.
Main Methods:
- Differential gene expression analysis using DESeq2.
- Gene Ontology (GO) enrichment analysis.
- Protein-protein interaction (PPI) network analysis.
Main Results:
- Identified significant changes in genes related to proliferation, cell adhesion, immune response, and transcriptional regulation.
- Discovered novel hub genes, including Il6 and Itgb2, alongside previously implicated genes like Mapk11 and Cdh1.
- Proposed a predicted gene regulatory network model for immortalized MEFs.
Conclusions:
- Spontaneous immortalization of MEFs involves widespread transcriptome reprogramming.
- The identified hub genes and regulatory network provide insights into the mechanisms driving immortalization and potential cancer development.
