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Updated: Jun 17, 2025

Pan-lyssavirus Real Time RT-PCR for Rabies Diagnosis
Published on: July 10, 2019
Real-Time RT-PCR Assays for Typing of Epizootic Hemorrhagic Disease Virus
Corinne Sailleau1, Stéphan Zientara2, Emmanuel Bréard2
1ANSES/INRA/ENVA-UPEC, Maisons Alfort, France. corinne.sailleau@anses.fr.
Abstract:
Real-time RT-PCR for the detection of epizootic hemorrhagic disease virus (EHDV) in clinical samples is a fast and sensitive tool for the diagnosis and confirmation of disease. Several real-time RT-PCR methods have been reported over the last 10 years. In this chapter, we describe seven duplex real-time RT-PCR assays to amplify part of genome segment 2 of EHDV to enable serotype identification. The assay includes the detection of an endogenous control gene-beta-actin.
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