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Protocol for fluorescent live-cell staining of tardigrades

Clayton J Harry1, Jonathan D Hibshman2, Amor Damatac3

  • 1Department of Biology, The University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA.

STAR Protocols
|August 11, 2024
PubMed
Abstract

Keywords:
Cell BiologyMicroscopyModel Organisms

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Fixation and Sectioning01:03

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Two basic types of preparation are used to visualize specimens with a light microscope: wet mounts and fixed specimens.
The simplest type of preparation is the wet mount, in which the specimen is placed in a drop of liquid on the slide. A liquid specimen can be directly deposited on the slide using a dropper. Solid specimens, such as skin scraping, can be placed on the slide before adding a drop of liquid to prepare the wet mount. Sometimes the liquid is simply water, but stains are often added...