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Acoustofluidic Virus Isolation via Bessel Beam Excitation Separation Technology.

Jianping Xia¹, Zeyu Wang¹, Ryan Becker²

¹The Thomas Lord Department of Mechanical Engineering and Materials, Duke University, Durham, North Carolina 27708, United States.

|August 12, 2024

Summary

We developed Bessel beam excitation separation technology (BEST) for rapid, high-resolution virus isolation from biological samples. This acoustofluidic method enhances viral RNA yield and purity for diagnostics and research.

Keywords:

Bessel beam COVID-19 SARS-CoV-2 acoustic separation nanoparticle viruses

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Area of Science:

Biotechnology
Virology
Acoustics

Background:

Virus isolation is crucial for developing viral therapeutics and vaccines, as highlighted during the COVID-19 pandemic.
Current viral isolation methods are often slow, labor-intensive, and yield low amounts of virus.

Purpose of the Study:

To introduce a novel, rapid, and efficient acoustofluidic technology for high-resolution virus isolation.
To demonstrate the capability of Bessel beam excitation separation technology (BEST) for isolating viruses from complex biological samples.

Main Methods:

Utilized 2D, in-plane acoustic Bessel beams for continuous separation of cell-free viruses from biofluids.
Employed Bessel beam excitation separation technology (BEST) for size-selective virus isolation by tuning acoustic parameters.
Applied BEST to isolate SARS-CoV-2 from saliva and Moloney Murine Leukemia Virus from cell culture media.

Main Results:

Achieved high throughput and high viral RNA yield in virus isolation.
Demonstrated dynamic, size-selective isolation, effectively removing larger and smaller contaminants.
Obtained high separation resolution, yield, and purity of isolated viruses.

Conclusions:

BEST offers a powerful tool for rapid and efficient virus isolation from complex biological samples.
This technology shows significant potential for advancing viral diagnostics, therapeutics, and vaccine development.
BEST is applicable to both practical diagnostic applications and fundamental virology research.