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Updated: Aug 8, 2026

Analysis of Dendritic Spine Morphology in Cultured CNS Neurons
Published on: July 13, 2011
Proteomic Identification and Label-Free Quantification of Proteins Implicated in Neurite and Spine Formation
Kanika Sharma1, Prashant Kaushal1, Vikas Kumar2,3
1Mass Spectrometry & Proteomics Core Facility, University of Nebraska Medical Center, Omaha, NE, USA.
Abstract:
The molecular mechanisms underlying neurite formation include multiple crosstalk between pathways such as membrane trafficking, intracellular signaling, and actin cytoskeletal rearrangement. To study the proteins involved in such complex pathways, we present a detailed workflow of the sample preparation for mass spectrometry-based proteomics and data analysis. We have also included steps to perform label-free quantification of proteins that will help researchers quantify changes in the expression levels of key regulators of neuronal morphogenesis on a global scale.
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