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Updated: Jul 23, 2026

Imaging Dendritic Spines of Rat Primary Hippocampal Neurons using Structured Illumination Microscopy
Published on: May 4, 2014
Imaging of Structural Plasticity of Dendritic Spines with Two-Photon Microscopy
Takeo Saneyoshi1, Yasunori Hayashi2
1Department of Life Science and Technology, School of Life Science and Technology, Tokyo Institute of Technology, Kanagawa, Japan. saneyoshi.t.aa@m.titech.ac.jp.
None:
Plasticity of synaptic transmission underlies learning and memory. It is accompanied by changes in the density and size of synapses, collectively called structural plasticity. Therefore, understanding the mechanism of structural plasticity is critical for understanding the mechanism of synaptic plasticity. In this chapter, we describe the procedures and equipment required to image structural plasticity of a single dendritic spine, which hosts excitatory synapses in the central nervous system, and underlying molecular interactions/biochemical reactions using two-photon fluorescence lifetime microscopy (2P-FLIM) in combination with Förster resonance energy transfer (FRET)-based biosensors.
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