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Updated: Jun 17, 2026

Assessing Neural Stem Cell Motility Using an Agarose Gel-based Microfluidic Device
Published on: February 11, 2008
Live Imaging Analysis of Axonal Regeneration in Human iPSC-Derived Motor Neurons Using a Microfluidic System
Katherine L Marshall1, Mohamed H Farah2
1Department of Neurology, Neuromuscular Division, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
Abstract:
Axonal damage is a common feature of traumatic injury and neurodegenerative disease. The capacity for axons to regenerate and to recover functionality after injury is a phenomenon that is seen readily in the peripheral nervous system, especially in rodent models, but human axonal regeneration is limited and does not lead to full functional recovery. Here we describe a system where dynamics of human axonal outgrowth and regeneration can be evaluated via live imaging of human-induced pluripotent stem cell (hiPSC)-derived neurons cultured in microfluidic systems, in which cell bodies are isolated from their axons. This system could aid in studying axonal outgrowth dynamics and could be useful for testing potential drugs that encourage regeneration and repair of the nervous system.
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