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Expression Analysis of VPS72 and Associated Biological Behaviors in Colon Cancer
Jun Cao1,2, Hao Zhang2, Xin Xie3
1Department of Oncology, The Third Affiliated Hospital of Xi'an Medical University, Xi'an, Shaanxi, 710061, People's Republic of China.
Background:
VPS72 is highly expressed in hepatocellular carcinoma and prostate cancer, participating in various cellular processes such as gene transcription, replication, DNA repair, maintenance of genome integrity, and cancer progression. However, its role in colorectal cancer remains unknown.
Methods:
Bioinformatic methods were used to analyze gene expression, correlation and patient survival. Western blotting, colony formation assays and animal experiments were used to evaluate the function of VPS72 in colon cancer in vivo and in vitro.
Results:
VPS72 was highly expressed in colon cancer tissues and correlated with poor overall survival (P<0.05) and relapse free survival (P<0.01). Furthermore, patients with III/IV clinical stage (P<0.001), N1 nodal metastasis (P<0.001) or N2 nodal metastasis (P<0.05) status had poor overall survival. Further analysis showed that VPS72 is correlated with proliferation and EMT biomarkers. Western blotting, colony formation assays and animal experiments showed that VPS72 overexpression promoted colon cancer proliferation and EMT progress.
Conclusion:
Our study found that VPS72 was correlated with poor overall survival in colon cancer patients, and high expressed level of VPS72 promoted colon cancer progression, indicating its role as a potential prognosis biomarker.
