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Therapy Testing in a Spheroid-based 3D Cell Culture Model for Head and Neck Squamous Cell Carcinoma
Published on: April 20, 2018
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A computational study of gene expression patterns in head and neck squamous cell carcinoma using TCGA data
Saqib Rauf1,2, Sami Ullah2, Muhammad Adil Abid2
1Institute of Integrative Biosciences, CECOS University Peshawar, 25000, Pakistan.
Future Science OA
|August 14, 2024
Summary
Head and Neck squamous cell carcinoma (HNSCC) is a significant cancer in Pakistan. This study identified key gene expression changes, like keratin family and S100A9, linked to HNSCC development and risk factors.
Area of Science:
- Oncology
- Genomics
- Molecular Biology
Background:
- Head and Neck squamous cell carcinoma (HNSCC) represents a major health concern, ranking as the second most prevalent cancer in Pakistan.
- Understanding the molecular underpinnings of HNSCC is crucial for developing effective diagnostic and therapeutic strategies.
Purpose of the Study:
- To identify differentially expressed genes (DEGs) in HNSCC.
- To explore the functional roles of these DEGs in tumor development.
- To investigate potential links between gene expression patterns and environmental risk factors in Pakistan.
Main Methods:
- Utilized gene expression data from The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) databases.
- Analyzed normal versus tumor samples to identify DEGs.
- Employed the Enrichr tool for Gene Ontology (GO) enrichment analysis to determine gene functions.
Main Results:
- Identified significant DEGs involved in HNSCC pathogenesis.
- Observed overexpression of the Keratin family genes and S100A9.
- Found underexpression of KRT4 and KRT13, which are involved in keratin protein production.
Conclusions:
- Gene expression profiles, including keratin family members and S100A9, are altered in HNSCC.
- Poor oral hygiene and smokeless tobacco use may contribute to oral stress, cellular damage, and subsequent cancer development.
- Further research into these DEGs could reveal novel therapeutic targets for HNSCC.
