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Development and tissue specific expression of RAPGEF1 (C3G) transcripts having exons encoding disordered segments

Archana Verma1,2, Abhishek Goel1, Niladri Koner1

  • 1CSIR-Centre for Cellular & Molecular Biology, Uppal Road, Habsiguda, Hyderabad, 500 007, India.

Molecular Biology Reports
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PubMed
Summary

Researchers discovered novel forms of the RAPGEF1 (C3G) protein, generated by alternative splicing. These new RAPGEF1 isoforms show varied expression in tissues and influence protein activity during development.

Keywords:
AlphaFoldAlternate splicingC3GDockingIntrinsically disordered regionLZerDRAPGEF1Serine-rich

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Area of Science:

  • Molecular Biology
  • Gene Regulation
  • Protein Isoforms

Background:

  • RAPGEF1 (C3G) is a Guanine nucleotide exchange factor crucial for mouse embryonic development.
  • It regulates gene expression and cytoskeletal organization, impacting cell proliferation and differentiation.
  • Previous studies predicted multiple RAPGEF1 transcripts, but their tissue-specific expression remained uninvestigated.

Purpose of the Study:

  • To investigate the detailed expression patterns of RAPGEF1 isoforms in various mouse tissues.
  • To identify novel RAPGEF1 transcripts and understand their role in protein regulation.

Main Methods:

  • Analysis of RAPGEF1 splicing hotspots, particularly involving exon-3 and exons 12-14.
  • Detection of unannotated transcripts using cassette exons in specific organs (heart, brain, testis, skeletal muscle).
  • In silico structural analysis (AlphaFold) and docking studies (LZerD) to predict functional impacts of isoforms.

Main Results:

  • Identified full-length RAPGEF1 isoforms arising from splicing at two major hotspots.
  • Discovered unpredicted transcripts incorporating cassette exons in heart, brain, testis, and skeletal muscle.
  • Observed isoform switching during differentiation of myocytes and embryonic stem cells; cassette exons encode a phosphorylatable, disordered peptide influencing protein interactions and catalytic activity.

Conclusions:

  • Demonstrated the expression of novel RAPGEF1 isoforms across different tissues and developmental stages.
  • Cassette exon inclusion represents a novel mechanism for regulating RAPGEF1 activity.
  • These findings provide insights into the complex regulation of RAPGEF1 function in various biological contexts.