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Related Concept Videos

Chromatin Immunoprecipitation- ChIP02:36

Chromatin Immunoprecipitation- ChIP

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Chromatin immunoprecipitation, or ChIP, is an antibody-based technique used to identify sites on DNA that bind to transcription factors of interest or histone proteins. It also helps determine the type of histone modifications such as acetylation, phosphorylation, or methylation.
Types of ChIP
ChIP can be divided into two types - X-ChIP and N-ChIP. X-ChIP involves in vivo cross-linking of histones and regulatory proteins to DNA, fragmenting the DNA by sonication, and isolating the protein-DNA...
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Related Experiment Video

Updated: Jun 17, 2025

Single-Cell Factor Localization on Chromatin using Ultra-Low Input Cleavage Under Targets and Release using Nuclease
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Single-Cell Histone Modification Profiling with Cell Enrichment Using sortChIC.

Helena Viñas Gaza1,2, Vivek Bhardwaj1,2, Peter Zeller3,4

  • 1Hubrecht Institute-KNAW (Royal Netherlands Academy of Arts and Sciences), Oncode Institute, Utrecht, The Netherlands.

Methods in Molecular Biology (Clifton, N.J.)
|August 14, 2024
PubMed
Summary
This summary is machine-generated.

Histone post-translational modifications (PTMs) can now be mapped in single cells using the novel sortChIC technique. This method enables the study of rare cell types and their dynamic chromatin changes during differentiation.

Keywords:
ChromatinEpigeneticsFACS sortingHistone modificationsSequencing dataSingle-cell ChIC-seq technique

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Automating ChIP-seq Experiments to Generate Epigenetic Profiles on 10,000 HeLa Cells
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Area of Science:

  • Epigenetics and Genomics
  • Cell Biology
  • Molecular Biology

Background:

  • Histone post-translational modifications (PTMs) regulate chromatin structure and gene expression.
  • Cell differentiation involves remodeling of histone modifications, creating cell type-specific patterns.
  • Studying these dynamic changes in rare, in vivo cell populations was previously limited by cell number requirements.

Purpose of the Study:

  • To present a sensitive single-cell method for mapping histone PTMs.
  • To enable the study of cell type-specific chromatin dynamics in complex biological systems.
  • To facilitate the analysis of rare cell types, such as stem and progenitor cells.

Main Methods:

  • Development of a sort-assisted single-cell Chromatin ImmunoCleavage sequencing technique (sortChIC).
  • Integration of histone PTM mapping with surface staining-based enrichment for rare cell isolation.
  • Application of sortChIC to quantify chromatin changes at the single-cell level.

Main Results:

  • sortChIC allows for the sensitive mapping of histone PTMs in individual cells.
  • The technique effectively enriches for rare cell populations, overcoming previous limitations.
  • Enables the study of chromatin remodeling in dynamic and heterogeneous cellular environments.

Conclusions:

  • sortChIC provides a powerful tool for investigating cell type-specific chromatin modifications.
  • This method facilitates research into lineage specification and cell-type determination in physiological and disease states.
  • Advances the understanding of epigenetics in complex biological systems at single-cell resolution.