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Protocol for in vivo nucleic acid delivery utilizing the rolling microneedle electrode array.

Pengfei Wu1, Tongren Yang2, Dong Huang3

  • 1School of Life Science, Advanced Research Institute of Multidisciplinary Science, Key Laboratory of Molecular Medicine and Biotherapy, Key Laboratory of Medical Molecule Science and Pharmaceutics Engineering, Beijing Institute of Technology, Beijing 100081, China.

STAR Protocols
|August 14, 2024
PubMed
Summary

We developed the rolling microneedle electrode array (RoMEA) for efficient, low-damage skin electroporation. This device enables enhanced nucleic acid delivery over large body areas.

Keywords:
biophysicsbiotechnology and bioengineeringmolecular biology

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Area of Science:

  • Biomedical Engineering
  • Molecular Biology
  • Dermatology

Background:

  • Electroporation is a method to increase cell membrane permeability for molecule absorption.
  • Current methods for electroporation can be inefficient or cause significant skin damage.

Purpose of the Study:

  • To develop and evaluate a novel device, the rolling microneedle electrode array (RoMEA), for efficient and low-damage electroporation.
  • To demonstrate the application of RoMEA for in vivo nucleic acid transfection over extended skin areas.

Main Methods:

  • Design, fabrication, and assembly of the RoMEA device, featuring a densely arranged microneedle electrode array with rolling structures.
  • Application of RoMEA for in vivo electroporation to create uniform skin micropores.
  • Transfection of nucleic acids into the skin using the RoMEA device.

Main Results:

  • The RoMEA device successfully created uniform skin micropores.
  • Efficient and low-damage transfection of nucleic acids was achieved over extended body areas.
  • Detailed protocol for device use and execution is provided.

Conclusions:

  • The rolling microneedle electrode array (RoMEA) is an effective tool for enhancing transdermal delivery of nucleic acids.
  • RoMEA offers a promising approach for efficient, large-area, low-damage electroporation in vivo.
  • This technology has potential applications in gene therapy and other transdermal delivery systems.