Aggregation-induced electrochemiluminescence based on intramolecular charge transfer and twisted molecular...

Organic emitters with exceptional properties exhibit significant potential in the field of aggregation-induced electrochemiluminescence (AIECL); however, their practicality is impeded by limited ECL efficiency (Φ_ECL). This paper investigates a novel type of AIECL emitter (BDPPA NPs), where an efficient intramolecular charge transfer (ICT) effect and highly twisted conformation contribute to a remarkable enhancement of ECL. The ICT effect reduces the electron transfer path, while the twisted conformation effectively restricts π-π stacking and intramolecular motions. Intriguingly, compared to the standard system of [Ru(bpy)₃²⁺]/TPrA, bright emissions with up to 54 % Φ_ECL were achieved, enabling direct visual observation of ECL through the co-reactant route. The label-free immunosensor exhibited distinguished performance in detecting SARS-CoV-2 N protein across an exceptionally wide linear range of 0.001-500 ng mL^-1, with a remarkably low detection limit of 0.28 pg mL^-1. Furthermore, this developed ECL platform exhibited excellent sensitivity, specificity, and stability characteristics, providing an efficient avenue for constructing platforms for bioanalysis and clinical diagnosis analysis.