Related Experiment Video
Updated: Jun 16, 2025
A Cost Effective and Adaptable Scratch Migration Assay
Published on: June 30, 2020
A cost-effective tool to standardize the scratch assay for cell migration
Cristina Bucchi1,2, Josefa Baeza1, Jaime Guarda3
1Integral Adult Dentistry Department, Universidad de La Frontera, Temuco, Chile.
Background:
The scratch assay is commonly used in cell biology to evaluate cell migration; however, it is not a standardized method; it produces highly variable gap dimensions. We design a printable device, comprising a single wounding tool and a guide, and compared the gap produced by our device and the traditional method. The deviceis printable in a standard 3D printer. Cells were seeded on a 24-well plate. After reaching full confluency, a gap was created using the traditional method (scratch assay with a pipette tip), a pipette tip and the guide of the device, or the single wounding tool and the guide. The gaps were observed for up to 48 h under a light microscope and analyzed.
Results:
The results show that the traditional method produces irregular and not straight gaps, and had the worst cell migration rates compared to the other groups. The wounding tool produced scrape signs at the well surface.
Conclusion:
The guide and pipette tip delivered the best results for the scratch assay.
Significance:
The use of the guide and the pipette tip for the scratch assay allows allows to perform reproducible cell migration experiments.
More Related Videos
05:27Optimized Scratch Assay for In Vitro Testing of Cell Migration with an Automated Optical Camera
Published on: August 8, 2018
08:40Optimization of the Wound Scratch Assay to Detect Changes in Murine Mesenchymal Stromal Cell Migration After Damage by Soluble Cigarette Smoke Extract
Published on: December 3, 2015
