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Genome-wide Association Studies-GWAS01:11

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Genome-wide association studies or GWAS are used to identify whether common SNPs are associated with certain diseases. Suppose specific SNPs are more frequently observed in individuals with a particular disease than those without the disease. In that case, those SNPs are said to be associated with the disease. Chi-square analysis is performed to check the probability of the allele likely to be associated with the disease.
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Every organism has an optimum temperature range within which healthy growth and physiological functioning can occur. At the ends of this range, there will be a minimum and maximum temperature that interrupt biological processes.
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Related Experiment Video

Updated: Jun 16, 2025

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Genome-wide association study for high-temperature tolerance in the Japanese flounder.

L Z San1, G X Wang1, Z W He1

  • 1State Key Laboratory of Mariculture Biobreeding and Sustainable Goods, Beidaihe Central Experiment Station, Chinese Academy of Fishery Sciences, Qinhuangdao 066100, China; Hebei Key Laboratory of the Bohai Sea Fish Germplasm Resources Conservation and Utilization, Beidaihe Central Experiment Station, Chinese Academy of Fishery Sciences, Qinhuangdao 066100, China; Bohai Sea Fishery Research Center, Chinese Academy of Fishery Sciences, Qinhuangdao 066100, China.

Animal : an International Journal of Animal Bioscience
|August 17, 2024
PubMed
Summary

Researchers identified genetic markers for high-temperature tolerance in Japanese flounder (Paralichthys olivaceus). This study provides tools for breeding more resilient fish, crucial for aquaculture survival and growth.

Keywords:
Candidate genesMolecular breedingSingle nucleotide polymorphismSurvival statusSurvival time

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Area of Science:

  • Aquaculture
  • Genetics
  • Animal Breeding

Background:

  • High-temperature stress poses a significant threat to Japanese flounder survival and aquaculture industry growth.
  • Developing Japanese flounder with enhanced heat tolerance is crucial for sustainable aquaculture.

Purpose of the Study:

  • To identify genetic markers linked to high-temperature tolerance in Japanese flounder.
  • To understand the genetic regulatory mechanisms underlying heat resistance.
  • To establish a foundation for selective breeding programs aimed at improving heat tolerance.

Main Methods:

  • A genome-wide association study (GWAS) was conducted on 280 Japanese flounder individuals.
  • High-quality single nucleotide polymorphisms (SNPs) were utilized to analyze genetic variations.
  • High-temperature tolerance was assessed by survival time and status at 31°C for 15 days.

Main Results:

  • Six significant loci on six chromosomes were associated with survival time under heat stress, with six candidate genes identified.
  • Thirty-four loci on 15 chromosomes were linked to survival status, and 22 candidate genes were annotated.
  • Genes such as traf4 and ppm1l were highlighted for their potential role in regulating apoptosis and heat tolerance.

Conclusions:

  • The study identified key genetic loci and candidate genes associated with high-temperature tolerance in Japanese flounder.
  • These findings offer a theoretical basis for incorporating molecular markers into breeding programs.
  • This research provides a molecular tool to enhance heat tolerance traits in cultured Japanese flounder, supporting aquaculture resilience.