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A novel structurally modified isophorone fluorescent probe for H2S detection.

Zhi Yuan Xie1, Wan Xiang Qiu1, Zi Yi Xu1

  • 1Key Laboratory of Luminescence Analysis and Molecular Sensing, School of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, PR China.

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A new fluorescent probe, SP-Br, was developed for sensitive detection of hydrogen sulfide (H2S). This probe offers improved performance, enabling effective monitoring in environmental and food samples.

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Area of Science:

  • Analytical Chemistry
  • Chemical Sensing
  • Biochemistry

Background:

  • Hydrogen sulfide (H2S) plays a crucial role in biological systems.
  • H2S is also found in environmental water and during food spoilage.
  • Sensitive detection methods for H2S are significant for various applications.

Purpose of the Study:

  • To design and synthesize a novel fluorescent probe for detecting hydrosulfide ions (H2S).
  • To evaluate the performance of the new probe compared to existing ones.
  • To demonstrate the probe's applicability in real-world samples.

Main Methods:

  • Synthesis of a new fluorescent probe, SP-Br (2-[3-[2-[3-bromo-4-(2,4- dinitrobenzenesulfonate)] ethenyl]-5,5-dimethyl-2-cyclohexen-1-ylidene]propanedinitrile).
  • Characterization of the probe's fluorescence properties and response to hydrosulfide ions.
  • Verification of the detection mechanism using high-resolution mass spectrometry, fluorescence, and UV-vis absorption spectroscopy.
  • Application testing in environmental water and beer samples.

Main Results:

  • SP-Br exhibits enhanced fluorescence emission at 670 nm in the presence of hydrosulfide ions.
  • The probe demonstrates a longer fluorescence emission, shorter response time (3 min), lower detection limit (149 nM), and wider detection range (0-30 nM) compared to similar probes.
  • Mechanism of H2S detection was confirmed through spectroscopic and mass spectrometry analyses.
  • Successful application of SP-Br for monitoring H2S in water and beer samples.

Conclusions:

  • SP-Br is a highly sensitive and efficient fluorescent probe for hydrosulfide ion detection.
  • The probe shows significant advantages over existing H2S sensors.
  • SP-Br has promising potential for environmental pollution monitoring and food quality analysis.