MiR-221 on protective oxidative induced by selenium modified Codonopsis pilosula polysaccharide

Area of Science:

• Molecular Biology
• Cellular Biology
• Biochemistry

Background:

• Oxidative stress is implicated in numerous diseases.
• MicroRNA-221 (miR-221) is known to modulate oxidative stress.
• The precise mechanism of miR-221 in sCPPS5-induced oxidative stress is not fully understood.

Purpose of the Study:

• To elucidate the protective effects of miR-221 against sCPPS5-induced oxidative stress.
• To investigate the underlying molecular mechanisms, particularly the Keap1-Nrf2-ARE signaling pathway.

Main Methods:

• Assessed oxidative stress markers: SOD, CAT, MDA, LDH, MMP, caspase-3 activity, and apoptosis.
• Utilized real-time PCR and Western blot to analyze Keap1-Nrf2-ARE pathway components.
• Evaluated effects in vivo using a mouse model.

Main Results:

• sCPPS5 treatment, particularly with miR-221 mimics, enhanced SOD and CAT expression.
• The Keap1-Nrf2-ARE signaling pathway was activated, leading to reduced MDA, MMP, caspase-3 activity, apoptosis, and LDH release.
• In vivo studies confirmed sCPPS5's ability to boost antioxidant enzymes and key signaling proteins.

Conclusions:

• miR-221 confers protection against sCPPS5-induced oxidative stress.
• This protective effect is likely mediated through the regulation of the Keap1-Nrf2-ARE signaling pathway in macrophages.
• Findings suggest a potential therapeutic role for miR-221 in managing oxidative stress-related conditions.