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Ionic liquid-based aqueous biphasic system as an alternative tool for enhanced bacterial DNA extraction
K K Athira1, Jijith Mepperi1, Hema Chandra Kotamarthi1
1Department of Chemistry, Indian Institute of Technology Madras, Chennai, 600036, India.
Analytica Chimica Acta
|August 18, 2024
Summary
This study introduces an eco-friendly, cost-effective DNA extraction method using Ammonium-based Ionic Liquids (ILs) in aqueous biphasic systems (ABS). The novel approach offers a benign alternative to conventional DNA extraction techniques.
Area of Science:
- Biochemistry
- Green Chemistry
- Molecular Biology
Background:
- Conventional DNA extraction methods are costly and potentially harmful.
- Ionic Liquids (ILs) show promise for DNA stabilization and processing.
- IL-based aqueous biphasic systems (ABS) offer a versatile platform for biomolecule extraction.
Purpose of the Study:
- To optimize an IL-based ABS for efficient and benign DNA extraction.
- To investigate the interactions between ILs and DNA for improved extraction.
- To develop a cost-effective and environmentally friendly DNA extraction protocol.
Main Methods:
- Designed an ABS using Ammonium-based ILs and Potassium phosphate buffer.
- Optimized biocompatible anions for DNA extraction efficiency.
- Validated the method for salmon sperm and plasmid DNA extraction from bacteria.
Main Results:
- Achieved DNA extraction results comparable to commercial kits.
- Demonstrated DNA stability in IL-rich phases.
- Confirmed successful DNA extraction via agarose gel electrophoresis and bacterial transformation.
Conclusions:
- Ammonium ILs form effective biphasic systems with various salting-out agents, unlike Cholinium ILs.
- Incorporation of biocompatible anions reduces IL toxicity.
- The developed IL-based ABS presents a cost-effective, eco-friendly alternative for DNA extraction.
