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3D Bioprinted Tissue-Engineered Bone with Enhanced Mechanical Strength and Bioactivities: Accelerating Bone Defect
Daqian Liu1,2, Jingsong Liu3, Pengcheng Zhao4
1Department of Orthopedic Surgery, The Second Affiliated Hospital of Harbin Medical University, Harbin Medical University, 246 Xuefu Road, Harbin, 150001, P. R. China.
Advanced Healthcare Materials
|August 19, 2024
Summary
A novel tissue-engineered bone material was developed to accelerate bone defect repair. This engineered bone temporally regulates immune responses, promoting healing for large bone defects.
Area of Science:
- Biomaterials Science
- Tissue Engineering
- Immunology
Background:
- Large bone defects present significant clinical challenges, often leading to delayed healing or nonunion.
- Current bone graft substitutes have limitations in promoting comprehensive bone regeneration and modulating the host immune response.
Purpose of the Study:
- To develop a novel, next-generation tissue-engineered bone with temporally controlled immunomodulatory properties.
- To address challenges in healing large bone defects by balancing pro- and anti-inflammatory responses.
Main Methods:
- Synthesized a photocurable methacrylated bone-derived decellularized extracellular matrix (bdECM-MA) hydrogel using multiphysics-assisted decellularization, biochemical modification, and freeze-drying.
- Fabricated the tissue-engineered bone by incorporating bdECM-MA with silicon-substituted calcium phosphate and bone marrow mesenchymal stem cells using digital light processing 3D bioprinting.
Main Results:
- In vitro studies confirmed high cellular viability and MPa-level mechanical strength of the engineered bone.
- Demonstrated excellent osteogenesis, angiogenesis, and immunomodulatory functions, including p38-MAPK pathway inhibition.
- In vivo studies revealed sequential pro- and anti-inflammatory responses, significantly accelerating bone defect repair.
Conclusions:
- The developed tissue-engineered bone effectively promotes bone regeneration and repair through controlled immunomodulation.
- This natural biomaterial-based approach offers a promising strategy for autogenous bone substitutes and treating large bone defects.
