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Related Experiment Video

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Omnidirectional anisotropic embedded 3D bioprinting.

Lei Shao1,2,3, Jinhong Jiang1,3, Chenhui Yuan1,4

  • 1Research Institute for Medical and Biological Engineering, Ningbo University, Ningbo, 315211, Zhejiang, China.

Materials Today. Bio
|August 19, 2024
PubMed
Summary
This summary is machine-generated.

This study introduces a novel 3D bioprinting method using shear-oriented bioink to create anisotropic, cell-laden artificial tissues. This technique overcomes limitations of conventional hydrogels, enabling precise fabrication of complex structures like blood vessels and muscle patches.

Keywords:
AnisotropyEmbedded 3D bioprintingMuscle patchShear-oriented bioink

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Area of Science:

  • Biomaterials Engineering
  • Tissue Engineering
  • Bioprinting Technology

Background:

  • Anisotropic microstructures are vital for physiological functions in human tissues like muscle and cornea.
  • Conventional hydrogels lack anisotropy, hindering the creation of complex, cell-laden anisotropic structures.
  • Direct embedded 3D cell-printing offers a promising approach for fabricating such structures.

Purpose of the Study:

  • To develop a direct embedded 3D bioprinting system for creating freeform anisotropic structures.
  • To utilize shear stress for orienting bioink components and cells during printing.
  • To enable the fabrication of intricate, cell-laden anisotropic artificial tissues with high precision.

Main Methods:

  • Development of a shear-oriented bioink (GelMA/PEO).
  • Establishment of an anisotropic embedded 3D bioprinting system utilizing extrusion-generated shear stress.
  • Incorporation of a carrageenan support bath for in-situ cell encapsulation and water-soluble PEO removal.
  • Fabrication of anisotropic blood vessel and muscle patch models.

Main Results:

  • Successfully created freeform anisotropic structures with controlled cell alignment using shear-oriented bioink.
  • Demonstrated high-precision, one-step bioprinting of intricate, porous, anisotropic artificial tissues.
  • Validated the system's effectiveness by fabricating anisotropic blood vessels and muscle patches.
  • Showcased the guiding role of anisotropic muscle patches on cell cytoskeleton extension.

Conclusions:

  • The developed anisotropic embedded 3D bioprinting system effectively produces cell-laden anisotropic tissues.
  • This technology overcomes limitations of isotropic hydrogels for fabricating complex anisotropic structures.
  • The method provides a significant foundation for ex-vivo and in-vivo applications of anisotropic artificial tissues.