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GhTTLL12 Coordinates With Transcriptional Regulators GhMML3 and GhMYB86 to Orchestrate Cotton Fibre Development
Xiaowei Ma1, Xubinmiao Huang1, Jinfang Li1,2
1Zhejiang Key Laboratory of Crop Germplasm Innovation & Utilization, The Advanced Seed Institute, Key Laboratory of Speed Breeding in Plant Factory (Ministry of Agriculture and Rural Affairs), College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, China.
Plant Biotechnology Journal
|June 24, 2026
Summary
Researchers identified GhTTLL12 as a key protein regulating cotton fibre development. This protein promotes cell division and elongation by influencing microtubules, offering insights for improving cotton fibre quality and yield.
Area of Science:
- Plant Biology
- Cell Biology
- Genetics
Background:
- Microtubules (MTs) are essential for plant cell processes like division and elongation.
- Cotton fibres, as single-celled trichomes, serve as a model for studying plant cell differentiation.
- The precise role of MTs in cotton fibre development requires further elucidation.
Purpose of the Study:
- To identify and characterize the function of GhTTLL12 in cotton fibre development.
- To investigate the molecular mechanisms by which GhTTLL12 regulates fibre initiation and elongation.
- To explore the potential of GhTTLL12 for improving cotton fibre quality.
Main Methods:
- Gh-Gb introgression analysis to identify GhTTLL12.
- Overexpression and CRISPR/Cas9 gene editing in cotton.
- MT co-sedimentation and immunofluorescence assays.
- Molecular mechanism investigation involving nuclear and cytoplasmic localization.
Main Results:
- GhTTLL12 positively regulates cotton fibre initiation and elongation.
- Overexpression of GhTTLL12 enhances plant growth and fibre length; knockout reduces fibre quality.
- GhTTLL12 directly binds to and promotes MT assembly and transverse array formation.
- A regulatory module involving GhMML3, GhMYB86, GhTTLL12, and GhTUB8 controls MT remodelling during fibre development.
Conclusions:
- GhTTLL12 is a crucial positive regulator of cotton fibre development, influencing both initiation and elongation.
- The study defines a novel regulatory module linking transcriptional control to MT dynamics for fibre development.
- Findings provide a basis for enhancing cotton fibre quality and yield through genetic manipulation.
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