Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Potentiometry: Types of Electrodes01:19

Potentiometry: Types of Electrodes

562
Reference electrodes serve as a stable reference point for potentiometric measurements, while indicator and working electrodes react to variations in the composition of a solution.
The Standard Hydrogen Electrode (SHE) is a widely used reference electrode that maintains zero potential across all temperatures. However, its need for a continuous hydrogen gas supply renders it impractical for everyday use.
An alternative to SHE is the Saturated Calomel Electrode (SCE). This electrode features an...
562
Amperometry: Overview01:10

Amperometry: Overview

479
Amperometry is a technique commonly used to measure the concentration of specific analytes in a solution by monitoring the electric current generated during an electrochemical reaction. It involves applying a constant potential between a working electrode and a reference electrode to measure the resulting current, which is proportional to the concentration of the analyte. The Clark oxygen electrode operates based on this principle of amperometry. It consists of a cathode and an anode enclosed...
479
Electrodes: Overview01:17

Electrodes: Overview

1.3K
 Electrochemical measurements are conducted in an electrochemical cell composed of various components that control and measure the current and potential. One fundamental component is electrodes, conductive materials that enable electron transfer reactions at their surfaces.
There are two main types of electrodes in electrochemical cells. The first type, known as the working or indicator electrode, has a potential that is sensitive to the analyte's concentration and reacts to changes in...
1.3K
Electrodeposition01:08

Electrodeposition

616
Electrodeposition is a technique used to separate an analyte from interferents by electrochemical processes. Here, the analyte is a metal ion that can be deposited on an electrode immersed in the sample solution. The electrochemical setup consists of an anode and a cathode. When an electric current is applied to the setup, oxidation occurs at the anode. At the cathode, which consists of a large metal surface, metal ions undergo reduction and deposit onto the surface.
Electrodeposition can...
616

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Molecular design of porphyrin precursors toward thermally stable microporous ordered carbonaceous frameworks for oxygen reduction reaction.

Nanoscale·2026
Same author

Surface-Engineered Ru-Graphene Mesosponge Catalysts for pH-Universal and Seawater Hydrogen Evolution.

ACS nanoscience Au·2026
Same author

Hollow-needle-like nanocarbon with chirality-discriminating inner walls.

Nanoscale·2026
Same author

Room Temperature Conversion of CO<sub>2</sub> Into Graphitic Carbon Quantum Dots by Field-Induced Electron Localization at Ag Nanoparticles/Electric Double Layer Interface.

Small methods·2026
Same author

Defect-Mediated Catalysis for Low-Temperature Formation of Graphene-Based Materials.

Journal of the American Chemical Society·2026
Same author

An amperometric l-citrulline biosensor.

Bioelectrochemistry (Amsterdam, Netherlands)·2026

Related Experiment Video

Updated: Jun 16, 2025

Anaerobic Protein Purification and Kinetic Analysis via Oxygen Electrode for Studying DesB Dioxygenase Activity and Inhibition
08:31

Anaerobic Protein Purification and Kinetic Analysis via Oxygen Electrode for Studying DesB Dioxygenase Activity and Inhibition

Published on: October 3, 2018

8.5K

Contamination-Free Reference Electrode Using Prussian Blue for Small Oxygen Sensors.

Akiko Yoshida1,2, Kritin Pirabul2, Shunsuke Fujii3

  • 1Techno Medica Co., Ltd., Yokohama, Kanagawa 224-0041, Japan.

ACS Applied Materials & Interfaces
|August 20, 2024
PubMed
Summary

This study introduces a novel Prussian blue-based reference electrode for small oxygen sensors, offering enhanced stability over traditional silver/silver chloride electrodes in point-of-care testing devices.

Keywords:
Prussian bluemesoporous carbonreference electrodescreen-printed devicesmall oxygen sensor

More Related Videos

Synthesis and Calibration of Phosphorescent Nanoprobes for Oxygen Imaging in Biological Systems
10:38

Synthesis and Calibration of Phosphorescent Nanoprobes for Oxygen Imaging in Biological Systems

Published on: March 3, 2010

13.9K
Electrochemical Roughening of Thin-Film Platinum Macro and Microelectrodes
08:32

Electrochemical Roughening of Thin-Film Platinum Macro and Microelectrodes

Published on: June 30, 2019

7.7K

Related Experiment Videos

Last Updated: Jun 16, 2025

Anaerobic Protein Purification and Kinetic Analysis via Oxygen Electrode for Studying DesB Dioxygenase Activity and Inhibition
08:31

Anaerobic Protein Purification and Kinetic Analysis via Oxygen Electrode for Studying DesB Dioxygenase Activity and Inhibition

Published on: October 3, 2018

8.5K
Synthesis and Calibration of Phosphorescent Nanoprobes for Oxygen Imaging in Biological Systems
10:38

Synthesis and Calibration of Phosphorescent Nanoprobes for Oxygen Imaging in Biological Systems

Published on: March 3, 2010

13.9K
Electrochemical Roughening of Thin-Film Platinum Macro and Microelectrodes
08:32

Electrochemical Roughening of Thin-Film Platinum Macro and Microelectrodes

Published on: June 30, 2019

7.7K

Area of Science:

  • Electrochemistry
  • Materials Science
  • Biomedical Engineering

Background:

  • Point-of-care testing (POCT) devices are crucial for efficient patient care.
  • Electrochemical sensing, common in POCTs like blood oxygen sensors, often relies on Ag/AgCl reference electrodes.
  • Ag/AgCl electrodes can contaminate working electrodes with silver ions, leading to device degradation.

Purpose of the Study:

  • To develop a stable and reliable reference electrode for small electrochemical POCT devices.
  • To address the limitations of conventional Ag/AgCl reference electrodes in POCT applications.

Main Methods:

  • Fabrication of a novel reference electrode using graphene-coated porous silica spheres (G/PSS) embedded with Prussian blue (PB), termed PB/G/PSS.
  • Dispersion of PB within a conductive G/PSS matrix to ensure electrolyte contact.
  • Screen printing of the PB/G/PSS electrode for POCT device fabrication.

Main Results:

  • The PB/G/PSS electrode demonstrated stable open-circuit potential within 30 mV for 31 days.
  • Oxygen sensors utilizing the PB/G/PSS electrode showed stable operation for several days.
  • Sensors with Ag/AgCl electrodes exhibited rapid deterioration within a day.

Conclusions:

  • The PB/G/PSS reference electrode offers significantly improved stability compared to Ag/AgCl electrodes.
  • This novel electrode is a promising alternative for small electrochemical-based POCT devices.
  • The screen-printable, stable PB/G/PSS electrode facilitates the development of next-generation POCTs.