Aptamer-Loop DNA Nanoflower Recognition and Multicolor Fluorescent Carbon Quantum Dots Labeling System for...

Area of Science:

• Biotechnology
• Molecular Biology
• Nanotechnology

Background:

• Visualizing multiple targets in living cells is crucial for understanding biological processes but faces challenges like complexity and cost.
• Existing methods often struggle with multiplexing and require expensive equipment.

Purpose of the Study:

• To develop an integrated nanoplatform using nucleic acid aptamers and DNA nanotechnology for advanced living cell imaging.
• To overcome limitations of current imaging techniques by offering a simple, cost-effective, and highly sensitive solution.

Main Methods:

• Synthesized aptamer-based recognition probes (RPs) via rolling circle amplification and self-assembled them into DNA nanoflowers.
• Created signal probes (SPs) by conjugating multicolor carbon quantum dots with oligonucleotides complementary to RPs.
• Developed aptamer-based imaging systems for individual/simultaneous imaging of PTK7, nucleolin, and ATP using base pairing hybridization.

Main Results:

• The developed system achieved specific recognition and efficient binding of cellular targets PTK7, nucleolin, and ATP.
• Demonstrated 5-10 fold signal amplification for enhanced fluorescence imaging and intensity analysis.
• Successfully tracked cell-cycle-dependent nucleolin distribution and concentration-dependent ATP fluorescence, validating its utility for monitoring cellular status.

Conclusions:

• The nanoplatform offers a simple, low-cost, highly selective, and sensitive approach for living cell imaging.
• This technology has significant potential for advancing biological process research and diagnostics.
• The system enables multiplexed imaging and real-time monitoring of cellular dynamics.