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Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization
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Off-axis digital lensless holographic microscopy based on spatially multiplexed interferometry.

José Ángel Picazo-Bueno1,2, Steffi Ketelhut1, Jürgen Schnekenburger1

  • 1University of Muenster, Biomedical Technology Center, Muenster, Germany.

Journal of Biomedical Optics
|August 20, 2024
PubMed
Summary

Lensless spatially multiplexed interferometric microscopy (LESSMIM) offers twin-image-free quantitative phase imaging for fast-moving biological samples. This compact, lens-free technique advances applications in areas like flow cytometry and sperm analysis.

Keywords:
digital holographic microscopydigital lensless holographic microscopylabel-free imagingoff-axis lensless holographyphase retrievalquantitative phase imagingspatially multiplexed interferometric microscopy

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Area of Science:

  • Biomedical Optics
  • Microscopy
  • Quantitative Phase Imaging

Background:

  • Digital holographic microscopy (DHM) provides label-free, time-resolved quantitative phase imaging (QPI) for biological samples.
  • Lensless DHM (DLHM) offers compact, cost-effective systems suitable for limited-resource settings.
  • In-line DLHM is limited by twin-image artifacts, hindering accurate QPI.

Purpose of the Study:

  • To develop a compact, lens-free, common-path interferometric off-axis approach for QPI of fast-moving biological specimens.
  • To overcome the twin-image limitation of in-line DLHM.

Main Methods:

  • Introduced lensless spatially multiplexed interferometric microscopy (LESSMIM), a lens-free variant of SMIM.
  • Utilized a common-path interferometric architecture with a single diffraction grating for digital off-axis holography.
  • Employed Fourier filtering, aberration compensation, and numerical propagation for twin-image-free QPI from single-shot holograms.

Main Results:

  • Demonstrated LESSMIM concept with a resolution test chart and assessed temporal stability.
  • Characterized QPI accuracy and imaging capabilities for living adherent cell cultures.
  • Evaluated cytometry of suspended cells in flow using a microfluidic channel.

Conclusions:

  • LESSMIM provides fast, time-resolved QPI in a compact optical setup, overcoming in-line DLHM limitations.
  • The technique is promising for biomedical applications requiring high-speed imaging, such as imaging flow cytometry and sperm cell analysis.