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Expression and pathogenesis of insulin-like growth factor-1 and insulin-like growth factor binding protein 3 in a
Weihua Wang1, Xuemei Sun2, Aina Wang3
1Department of Gastroenterology, The Affiliated Hospital of Qingdao University, Shandong, China.
Background And Aim:
Insulin-like growth factor-1 may be involved in the epithelial-to-mesenchymal transition process. It can mitigate adverse effects when interacting with insulin-like growth factor binding protein 3. This study aimed to explore alterations in the expression of these two factors in the colonic tissue of mice with ulcerative colitis.
Method:
This study utilized animal models. Mice were randomly allocated into three distinct groups. Disease activity index assessment was performed first, followed by histological grading of colitis. Protein and mRNA expression levels were determined using Western blotting and RT-qPCR. Immunohistochemical detection was used to determine histochemistry scores. Pearson correlation and SPSS 25.0 software were used for data analysis.
Results:
The findings indicated a reduction in the expression of the two investigated factors as well as in epithelial-to-mesenchymal transition epithelial markers during inflammation, while the expression of noninflammatory factors increased. These effects were notably amplified following treatment. Interestingly, the changes in epithelial-to-mesenchymal transition-inducing factors and mesenchymal markers contradicted this trend. Pearson correlation analysis revealed a correlation between molecular indicators of change and epithelial-to-mesenchymal transition.
Conclusion:
Insulin-like growth factor-1 and insulin-like growth factor binding protein 3 may play a protective role in the development and progression of ulcerative colitis, potentially through their inhibition of the epithelial-to-mesenchymal transition. These factors hold promise as targets for the clinical diagnosis and treatment of ulcerative colitis.
Insights
Insulin-like growth factor-1 and insulin-like growth factor binding protein 3 may protect against ulcerative colitis by inhibiting epithelial-to-mesenchymal transition. These factors show promise for diagnosis and treatment.
Area of Science:
- Gastroenterology and Molecular Biology
Background:
- Epithelial-to-mesenchymal transition (EMT) is implicated in ulcerative colitis pathogenesis.
- Insulin-like growth factor-1 (IGF-1) and its binding protein 3 (IGFBP-3) may modulate EMT.
Purpose of the Study:
- To investigate alterations in IGF-1 and IGFBP-3 expression in the colonic tissue of mice with induced ulcerative colitis.
- To explore the relationship between IGF-1, IGFBP-3, and EMT markers in ulcerative colitis.
Main Methods:
- Animal model of ulcerative colitis.
- Assessment of disease activity index and histological grading.
- Quantification of protein and mRNA expression using Western blotting and RT-qPCR.
- Immunohistochemical analysis for histochemistry scores.
- Statistical analysis using Pearson correlation and SPSS software.
Main Results:
- Reduced expression of IGF-1, IGFBP-3, and EMT epithelial markers during colitis.
- Increased expression of noninflammatory factors during colitis, amplified post-treatment.
- Contradictory trends observed for EMT-inducing factors and mesenchymal markers.
- Correlation found between molecular changes and EMT.
Conclusions:
- IGF-1 and IGFBP-3 may exert a protective effect in ulcerative colitis by inhibiting EMT.
- These factors present potential as biomarkers for clinical diagnosis and therapeutic targets for ulcerative colitis.
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