Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Measuring Reaction Rates03:09

Measuring Reaction Rates

24.9K
Polarimetry finds application in chemical kinetics to measure the concentration and reaction kinetics of optically active substances during a chemical reaction. Optically active substances have the capability of rotating the plane of polarization of linearly polarized light passing through them—a feature called optical rotation. Optical activity is attributed to the molecular structure of substances. Normal monochromatic light is unpolarized and possesses oscillations of the electrical...
24.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Influence of patients' health beliefs on rehabilitation exercise adherence after knee replacement: the mediating role of exercise psychological needs satisfaction.

Scientific reports·2026
Same author

Single-molecule readout of reversible nanoswitches enables continuous monitoring of low biomarker concentrations.

Nature communications·2026
Same author

Continuous Sensing of Lactate Dehydrogenase with a Competitive Particle-Based Sensor for Monitoring Cell Death.

ACS sensors·2026
Same author

Simulation-guided exploration of PAINT parameter space for accurate molecular quantification.

Nanoscale·2025
Same author

Reversible Sandwich-Based Particle Nanoswitch for Continuous Protein Monitoring at Picomolar Concentrations with Automated Calibration.

Angewandte Chemie (International ed. in English)·2025
Same author

Spatial Molecular Heterogeneity on Biofunctionalized Particles Quantified by Three-Dimensional Single-Molecule DNA-PAINT.

Langmuir : the ACS journal of surfaces and colloids·2025

Related Experiment Video

Updated: Jun 16, 2025

Microfluidic Platform with Multiplexed Electronic Detection for Spatial Tracking of Particles
11:54

Microfluidic Platform with Multiplexed Electronic Detection for Spatial Tracking of Particles

Published on: March 13, 2017

9.3K

Continuous Biosensor Based on Particle Motion: How Does the Concentration Measurement Precision Depend on Time Scale?

Rafiq M Lubken1, Yu-Ting Lin1, Stijn R R Haenen1

  • 1Helia Biomonitoring, Eindhoven 5612 AR, The Netherlands.

ACS Sensors
|August 21, 2024
PubMed
Summary

Understanding measurement imprecision is key for continuous biosensors. This study quantizes imprecision sources for Biosensing by Particle Motion (BPM) sensors, revealing sensor noise as the primary variation factor.

Keywords:
affinity-based sensinganalytical performancecontinuous biosensingcontinuous monitoringmeasurement precision

More Related Videos

A Protocol for Real-time 3D Single Particle Tracking
10:16

A Protocol for Real-time 3D Single Particle Tracking

Published on: January 3, 2018

14.9K
Mass-Sensitive Particle Tracking to Characterize Membrane-Associated Macromolecule Dynamics
13:30

Mass-Sensitive Particle Tracking to Characterize Membrane-Associated Macromolecule Dynamics

Published on: February 18, 2022

4.0K

Related Experiment Videos

Last Updated: Jun 16, 2025

Microfluidic Platform with Multiplexed Electronic Detection for Spatial Tracking of Particles
11:54

Microfluidic Platform with Multiplexed Electronic Detection for Spatial Tracking of Particles

Published on: March 13, 2017

9.3K
A Protocol for Real-time 3D Single Particle Tracking
10:16

A Protocol for Real-time 3D Single Particle Tracking

Published on: January 3, 2018

14.9K
Mass-Sensitive Particle Tracking to Characterize Membrane-Associated Macromolecule Dynamics
13:30

Mass-Sensitive Particle Tracking to Characterize Membrane-Associated Macromolecule Dynamics

Published on: February 18, 2022

4.0K

Area of Science:

  • Biosensing technologies
  • Analytical chemistry
  • Statistical analysis of measurement data

Background:

  • Continuous biosensors enable long-term monitoring of biomolecular substances.
  • Accurate interpretation of concentration-time profiles requires understanding measurement imprecision over various time scales.
  • Quantifying imprecision sources is crucial for advancing continuous sensing technologies.

Purpose of the Study:

  • To define and analyze measurement imprecisions for continuous biosensing, considering different variation sources and time scales.
  • To exemplify a methodology for quantifying imprecision using Biosensing by Particle Motion (BPM) technology.
  • To identify key factors contributing to measurement imprecision in a real-world application.

Main Methods:

  • Statistical analysis of extensive measurement data from nearly 50 BPM sensor cartridges over several months.
  • Development of a methodology to extract and compare imprecision parameters related to different variation sources and time scales (minutes to weeks).
  • Application of the methodology to a BPM sensor measuring glycoalkaloids in potato fruit juice.

Main Results:

  • Relative residuals of the BPM sensor measurements were found to be normally distributed.
  • Sensor noise was identified as the most significant source of variation, followed by sample pretreatment.
  • Variations from fluidics, sensor drift, and cartridge differences were found to be minor.

Conclusions:

  • The developed methodology effectively elucidates time-dependent and time-independent factors in measurement imprecision.
  • Sensor noise, stemming from stochastic fluctuations and nonspecific interactions, is the dominant imprecision factor on short time scales.
  • The findings provide essential knowledge for interpreting continuous biosensor data and for future sensor development.