Fabrication of Glucose Fluorescent Aptasensor Based on CdTe Quantum Dots

Area of Science:

• Biomedical Engineering
• Analytical Chemistry
• Nanotechnology

Background:

• Diabetes mellitus is a chronic metabolic disorder characterized by hyperglycemia, necessitating precise glucose monitoring to prevent severe health complications.
• Effective glucose monitoring is crucial for managing diabetes, reducing symptoms, and avoiding long-term damage to vital organs.

Purpose of the Study:

• To develop a simple, rapid, sensitive, and cost-effective fluorescent aptasensor for accurate glucose determination.
• To create a novel sensing platform utilizing quantum dots for enhanced glucose detection.

Main Methods:

• Fabrication of a fluorescent aptasensor using cadmium telluride quantum dots (CdTe QDs) functionalized with thiol-glucose-aptamer.
• Characterization of the aptasensor using UV-visible spectroscopy, FT-IR, FESEM, EDX, TEM, AFM, and DLS.
• Evaluation of sensor performance including detection limit, relative standard deviation, specificity, and stability.

Main Results:

• The aptasensor exhibited a linear relationship between fluorescence quenching and glucose concentration, with a low detection limit (0.13 ± 1.95 × 10⁻¹¹ mol L⁻¹) and high precision (RSD 1.05%).
• The sensor demonstrated excellent specificity for glucose and maintained stability for over 28 days.
• Successful detection of glucose in human serum and urine samples with a recovery rate up to 99.74%.

Conclusions:

• The developed fluorescent aptasensor is a promising tool for robust sensing technology, particularly for low-concentrated analytes like glucose.
• This technology offers a valuable platform for improved diabetes monitoring and management.