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Related Concept Videos

Photoluminescence: Applications01:14

Photoluminescence: Applications

385
Photoluminescence offers a wide range of applications due to its inherent sensitivity and selectivity. This technique allows for both direct and indirect analyses of the analyte. Direct quantitative analysis is possible when the analyte exhibits a favorable quantum yield for fluorescence or phosphorescence. However, an indirect analysis may be feasible if the analyte is not fluorescent or phosphorescent, or if the quantum yield is unfavorable. Indirect methods include reacting the analyte with...
385

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Related Experiment Video

Updated: Jun 15, 2025

Author Spotlight: Engineering Molecular Tools for Disease Detection and Imaging
04:33

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Published on: December 8, 2023

798

Cu2Cl(OH)3 nanozyme-based colorimetric sensor array for phosphates discrimination and disease identification.

Qihao Shi1, Yu Wang1, Qingfu Zhang1

  • 1Tianjin Key Laboratory of Industrial Microbiology, Tianjin University of Science and Technology, No.29 of 13th Street, TEDA, Tianjin, 300457, PR China.

Talanta
|August 21, 2024
PubMed
Summary

This study introduces a novel nanozyme for detecting multiple phosphates, crucial for physiological processes and disease diagnosis. The developed sensor array accurately distinguishes eight different phosphates in complex samples.

Keywords:
Colorimetric sensor arrayDeep eutectic solventsDiscriminationNanozymePhosphates

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Area of Science:

  • Biochemistry
  • Nanotechnology
  • Analytical Chemistry

Background:

  • Accurate detection and differentiation of phosphates are vital for understanding physiological processes and diagnosing diseases.
  • Traditional methods face challenges in simultaneously identifying and distinguishing various phosphates due to synthesis complexities.
  • Developing efficient sensor arrays for phosphate analysis remains a significant challenge in analytical chemistry.

Purpose of the Study:

  • To synthesize a bifunctional nanozyme with dual enzyme-mimicking activities for phosphate detection.
  • To design a sensor array leveraging the unique properties of the nanozyme for identifying multiple phosphates.
  • To demonstrate the capability of the sensor array in distinguishing phosphates in complex biological samples.

Main Methods:

  • Synthesis of a bifunctional dicopper chloride trihydroxide (Cu2Cl(OH)3) nanozyme using basic deep eutectic solvents (DES).
  • Exploitation of the laccase-like and peroxidase-like activities of the nanozyme, modulated by different phosphates.
  • Development of a sensor array based on the differential responses of the nanozyme's activities to various phosphates.

Main Results:

  • The Cu2Cl(OH)3 nanozyme exhibited significant laccase- and peroxidase-like activities.
  • A sensor array was successfully constructed and utilized for the identification of eight distinct phosphates (ATP, ADP, AMP, PPi, Pi, GTP, GDP, and GMP).
  • The sensor array accurately distinguished between three simulated clinical samples representing different health statuses.

Conclusions:

  • The developed bifunctional nanozyme and sensor array offer a streamlined approach for constructing array channels.
  • This method significantly enhances the discrimination of multiple phosphates in intricate and real-world samples.
  • The findings contribute to advancing sensitive and selective phosphate detection for diagnostic applications.