Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Renewal of Intestinal Stem Cells01:23

Renewal of Intestinal Stem Cells

2.5K
The intestinal epithelial lining rapidly renews every 4 to 5 days. The renewal is facilitated by intestinal stem cells (ISCs) located at the base of the crypt– a gland located at the bottom of each villus. ISCs divide asymmetrically to form new stem cells and progenitor daughter cells. The daughter cells are called transit-amplifying (TA) cells which move upwards along the crypt and either differentiate into absorptive cells– the enterocytes or secretory cells– including the...
2.5K
Role Of Notch Signalling In Intestinal Stem Cell Renewal01:12

Role Of Notch Signalling In Intestinal Stem Cell Renewal

2.1K
Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
2.1K
Role of Ephrin-Eph Signalling in Intestinal Stem Cell Renewal01:22

Role of Ephrin-Eph Signalling in Intestinal Stem Cell Renewal

2.2K
Erythropoietin-producing hepatocellular carcinoma receptor (Eph) and its ligand, Eph receptor-interacting protein (Ephrin) were first discovered in the human carcinoma cell line, hence the name. Ephrin-Eph interaction guides cells to reach their appropriate location in adult tissues. They also play an essential role in the immune system by helping in immune cell migration, adhesion, and activation. Based on their structure and function, Eph is divided into two classes — EphA and EphB.
2.2K
Adult Stem Cells01:33

Adult Stem Cells

28.1K
Stem cells are undifferentiated cells that divide and produce more stem cells or progenitor cells that differentiate into mature, specialized cell types. All the cells in the body are generated from stem cells in the early embryo, but small populations of stem cells are also present in many adult tissues including the bone marrow, brain, skin, and gut. These adult stem cells typically produce the various cell types found in that tissue—to replace cells that are damaged or to continuously...
28.1K
Multipotency of Hematopoietic Stem Cells01:19

Multipotency of Hematopoietic Stem Cells

3.0K
The hematopoietic stem cells or HSCs are multipotent, meaning they can differentiate and give rise to all blood and immune cells. HSCs are maintained in the quiescent stage until an external stimulus initiates their differentiation. The multipotent HSCs exist as two heterogeneous populations, long-term repopulating cells (LTRC) and short-term repopulating cells (STRC). The two HSC populations have different surface markers or receptors and are classified based on quiescence and long-term...
3.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Ketogenic diet mediates intestinal tumorigenesis through lipids not ketones.

Nature·2026
Same author

A high-fat, low-carbohydrate diet can feed intestinal tumours.

Nature·2026
Same author

<i>Clostridioides difficile</i> Stimulates <i>CCL20</i> Expression in Human Colonoid Monolayers in a Transwell-Based Coculture System That Supports Its Anaerobic Growth.

International journal of microbiology·2026
Same author

Impact of vitamin D on the colon cancer immune microenvironment: results of a randomized clinical trial of preoperative vitamin D supplementation in patients with stage I-III colon cancer.

Cancer discovery·2026
Same author

Correction: <sup>1</sup>H-NMR serum metabolomic profiling from clinical routine identifies signatures of progressive melanoma metastasis.

Scientific reports·2026
Same author

Small intestine microbiota development prevents early-life adiposity via IL-22-mediated intestinal PPARα suppression.

bioRxiv : the preprint server for biology·2026

Related Experiment Video

Updated: Jun 15, 2025

Intestinal Epithelial Regeneration in Response to Ionizing Irradiation
09:10

Intestinal Epithelial Regeneration in Response to Ionizing Irradiation

Published on: July 27, 2022

2.2K

Short-term post-fast refeeding enhances intestinal stemness via polyamines.

Shinya Imada1, Saleh Khawaled1, Heaji Shin1

  • 1Department of Biology, The David H. Koch Institute for Integrative Cancer Research at MIT, MIT, Cambridge, MA, USA.

Nature
|August 21, 2024
PubMed
Summary

Refeeding after fasting boosts intestinal stem cell regeneration and tumor formation by activating mTORC1 and polyamine metabolism. This highlights potential cancer risks in diet-based regeneration strategies.

More Related Videos

Intestinal Stem Cell Isolation and Culture in a Porcine Model of Segmental Small Intestinal Ischemia
08:55

Intestinal Stem Cell Isolation and Culture in a Porcine Model of Segmental Small Intestinal Ischemia

Published on: May 18, 2018

10.8K
Real Time Analysis of Metabolic Profile in Ex Vivo Mouse Intestinal Crypt Organoid Cultures
08:53

Real Time Analysis of Metabolic Profile in Ex Vivo Mouse Intestinal Crypt Organoid Cultures

Published on: November 3, 2014

16.2K

Related Experiment Videos

Last Updated: Jun 15, 2025

Intestinal Epithelial Regeneration in Response to Ionizing Irradiation
09:10

Intestinal Epithelial Regeneration in Response to Ionizing Irradiation

Published on: July 27, 2022

2.2K
Intestinal Stem Cell Isolation and Culture in a Porcine Model of Segmental Small Intestinal Ischemia
08:55

Intestinal Stem Cell Isolation and Culture in a Porcine Model of Segmental Small Intestinal Ischemia

Published on: May 18, 2018

10.8K
Real Time Analysis of Metabolic Profile in Ex Vivo Mouse Intestinal Crypt Organoid Cultures
08:53

Real Time Analysis of Metabolic Profile in Ex Vivo Mouse Intestinal Crypt Organoid Cultures

Published on: November 3, 2014

16.2K

Area of Science:

  • Cell Biology
  • Gastroenterology
  • Oncology

Background:

  • Fasting regimens are known to improve health, lifespan, and tissue regeneration.
  • The impact of fasting and refeeding on adult stem cells and tumor formation remains underexplored.

Purpose of the Study:

  • To investigate how post-fast refeeding affects intestinal stem cell (ISC) proliferation and tumor formation.
  • To elucidate the molecular mechanisms driving these effects.

Main Methods:

  • Studied the effects of post-fast refeeding on Lgr5+ intestinal stem cells (ISCs) in mice.
  • Investigated the role of mTORC1 signaling, polyamine metabolism, and protein synthesis.
  • Assessed tumor incidence in the context of Apc gene mutation.

Main Results:

  • Post-fast refeeding significantly increases ISC proliferation and tumor formation.
  • This effect is mediated by mTORC1 induction, enhanced protein synthesis via polyamine metabolism.
  • Loss of the Apc tumor suppressor gene in refed ISCs leads to higher tumor incidence.

Conclusions:

  • Post-fast refeeding represents a distinct physiological state that promotes stem cell regeneration and tumorigenesis.
  • Diet-based strategies involving fasting and refeeding require careful consideration to mitigate cancer risk.
  • Targeting mTORC1, polyamine metabolism, or protein synthesis may offer therapeutic avenues.