A recoverable hydrophilic deep eutectic solvent for efficient extraction of polyphenolic compounds from raspberry...

Area of Science:

• Green Chemistry
• Natural Product Chemistry
• Biotechnology

Background:

• Deep eutectic solvents (DESs) are effective for plant bioactive compound extraction.
• Limited development exists for hydrophilic DES and efficient isolation techniques for plant-derived compounds.

Purpose of the Study:

• To synthesize a hydrophilic DES (CL-CA-DES) from citric acid and choline chloride.
• To develop an integrated method for extracting and isolating polyphenolic compounds from raspberry roots using the synthesized DES and ion-exchange chromatography.
• To evaluate the recovery efficiency of the hydrophilic DES.

Main Methods:

• Synthesis of choline chloride-citric acid DES (CL-CA-DES).
• Extraction of polyphenolic compounds (catechins, epicatechins, procyanidin B1, procyanidin B2) from raspberry root using CL-CA-DES.
• Isolation of compounds via anion- and cation-exchange resin column chromatography.
• Assessment of DES recovery rate.

Main Results:

• CL-CA-DES demonstrated superior or comparable extraction efficiency for target polyphenols compared to conventional solvents.
• Ion-exchange chromatography provided higher isolation efficiency than organic solvent extraction and precipitation.
• The hydrophilic DES (CL-CA-DES) was recovered with a high efficiency exceeding 60%.

Conclusions:

• A novel, efficient, and environmentally friendly method for isolating polyphenolic compounds from raspberry roots was developed.
• The integrated approach utilizes a recyclable hydrophilic DES and ion-exchange chromatography.
• This method offers a sustainable alternative for natural product extraction and purification.